FAP-1在5-azaC诱导的HSG细胞凋亡中的作用  

Role of FAP-1 in apoptosis of human salivary gland cells inducted by 5-azacytidine

作　　者：白忠诚[1] 司徒镇强[2] 李莉莉[2] 张艳茹[1] 施生根[1] 

机构地区：[1]解放军第306医院全军口腔疾病诊治中心,北京100101 [2]第四军医大学口腔医学院

出　　处：《现代口腔医学杂志》2009年第6期634-636,共3页Journal of Modern Stomatology

摘　　要：目的体外研究FAP-1在5-氮胞苷(5-azacytidine,5-azaC)诱导的HSG(human salivary gland,HSG,人涎腺导管细胞,起源于人颌下腺闰管细胞)细胞凋亡中的作用,为应用5-azaC对涎腺肿瘤进行治疗奠定理论和实验基础。方法HSG细胞转染FAP-1反义寡核苷酸前后分别用5-azaC处理,观察转染前后FAP-1表达的变化,同时观察5-azaC对转染前后细胞凋亡作用是否发生变化。结果在形态上转染后的细胞与转染前无明显区别,转染FAP-1反义寡核苷酸链的HSG细胞FAP-1表达较转染前减弱,转染后HSG细胞对5-azaC比转染前敏感,加入5umol/L5-azaC后,凋亡细胞数增加。结论FAP-1在5-azaC诱导的HSG细胞凋亡中起一定作用。Objective To investigate the role of FAP - 1 in apoptosis of human salivary gland coils （HSG） inducted by 5 - azacytidine （5 - azaC） . Methods HSG ceils transfected by FAP - 1 antisense oligonucleotides were treated with 5 - azaC. The morphology of HSG and expression of FAP - 1 was then examined. Results The configuration of HSG ceils did not change after transfected by FAP - 1 antisense oligonucleotides, compared with HSG ceils that not transfected by. The expression of FAP - 1 was knock - down after transfected by FAP - 1 antisense oligonucleotides. More HSG colls underwent apoptosis when 5umol/L 5 - azaC was added after transfected by FAP - 1 antisense oligonudeotide. Conclusion FAP - 1 play an important role in the apoptosis effect of 5 ~ azaC on HSG cell in vitro.

关 键 词：FAP-1 HSG细胞 凋亡 

分 类 号：R73-3[医药卫生—肿瘤]