灵芝多糖对2型糖尿病大鼠胸主动脉内皮功能的保护作用  

Protective Effects of Ganoderma Lucidum Polysaccharides on Aorta Pectoralis Endothelial in Type 2 Diebetes Rats in Vivo

作　　者：乔进[1] 薛华[1] 孟国梁[1] 吴锋[1] 陈杨[1] 陈惠[2] 郑惠华[2] 徐济良[1] 

机构地区：[1]南通大学医学院药理学教研室,江苏省南通市226001 [2]江苏安惠生物科技有限公司,江苏省南通市226006

出　　处：《中国动脉硬化杂志》2009年第9期709-713,共5页Chinese Journal of Arteriosclerosis

基　　金：科技部十一五国家科技支撑计划子课题(2006DAI06A20-02);南通大学校级自然科学研究项目(08Z047);南通大学研究生科技创新计划项目(YKC08020)

摘　　要：目的研究灵芝多糖在体内对糖尿病大鼠主动脉内皮功能的影响,并探讨灵芝多糖保护血管内皮的可能机制。方法SD大鼠经过4周高脂饮食后腹腔注射链脲佐菌素(30 mg/kg)造模随机分为正常对照组、模型组、小檗碱组、灵芝多糖低、中及高剂量组(剂量分别为200、400和800 mg/kg)。16周给药治疗后,切取胸主动脉,制成3 mm血管环,检测乙酰胆碱引起的内皮依赖性舒张反应,并测定血清中糖基化终末产物含量、过氧化氢酶、谷胱甘肽过氧化物酶、总胆固醇和甘油三酯水平。结果灵芝多糖中剂量组和高剂量组血糖浓度较糖尿病模型组相比明显下降(P<0.01)。灵芝多糖高剂量组血清糖基化终末产物、总胆固醇和甘油三酯含量较模型组相比明显下降(P<0.01),而血清过氧化氢酶水平和谷胱甘肽过氧化物酶水平则明显上升(P<0.01)。结论灵芝多糖对糖尿病大鼠主动脉内皮依赖性舒张反应的损害有明显的保护作用,其机制可能与抑制血清糖基化终末产物、增强机体内过氧化氢酶和谷胱甘肽过氧化物酶活性和调节血脂障碍有关。Aim To investigate the effects of ganoderma lucidum polysaccharide on endothelial function of type 2 diabetic rats in vivo, and explore the potential mechanisms of ganoderma lucidum polysaccharide on protecting the endoderre.is of blood vessel. Methods SD rats were fed with high fat diet for 4 weeks, then injected STZ （ 30 mg/kg） to replicate type 2 diabetic rats. The successful diabetic rats model were randomized into normal control group, diabetes group, berberine （ 30 mg/kg） in the positive control group, ganoderma lucidum polysaccharide of low, middle and high-dose group （Ganoderma hicidum polysaccharide were orally given 200 mg/kg, 400 mg/kg and 800 mg/kg）. After 16 weeks＇ treatment, the thoracic aorta was immediately isolated. Endothelium-dependent relaxation induced by acetylcholine, the activity of eatalase （ CAT） , glutathione peroxidase （ GSH-PX ）, total cholesterol （ TC ） and triglycefides （ TG ） in serum were detected. Results After treatment the blood glucose concentration of middle-dose group and high-dose group was lower than diabetes group （ P 〈 0.01 ）. The advanced glycation end product （ AGE ） in serum was significantly lower in highdose group than diabetes group （ P 〈 0.01 ）, eatalase and glutathione peroxidase in serum was significantly higher in highdose group than diabetes group （P〈0.01）, TC and TG in serum was significantly lower in high-dose group than diabetes group （P 〈 0.01 ）. Condusion Ganoderma lucidum polysaeeharide is able to protect against aorta endothelial dysfunction in diabetic rats. The mechanisms may be involved in decreaseing advanced glycation end products in serum, enhancing the activity of CAT and GSH-PX in serum and accommodating dyslipidemia.

关 键 词：灵芝多糖 内皮依赖性舒张 糖基化终末产物 氧化应激 动脉粥样硬化 

分 类 号：R96[医药卫生—药理学]