胃癌中高表达的miR-21对胃癌细胞生物学行为的影响  

Influence of high miR-21 expression on biological behavior of gastric cancer cell

作　　者：张磊[1] 李建芳[1] 蔡劬[1] 陈雪华[1] 刘炳亚[1] 朱正纲[1] 燕敏[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院外科,上海消化外科研究所,上海200025

出　　处：《诊断学理论与实践》2009年第5期520-525,共6页Journal of Diagnostics Concepts & Practice

基　　金：上海市科学技术委员重点基础项目(07jc14041);上海市重点学科(s30204);国家自然科学基金资助项目(30872476)

摘　　要：目的:研究miR-21在胃癌中的表达情况及其对胃癌细胞生物学行为的影响。方法:通过实时荧光定量PCR检测30例胃癌手术切除组织和周围正常组织中以及10株胃癌细胞中miR-21的表达情况。通过CCK8法、Annexin-Ⅴ+PI、流式细胞仪检测、划痕实验,观察过表达miR-21对胃癌细胞增殖、凋亡、细胞周期、迁移等生物学行为的影响。结果:定量real-time PCR结果显示miR-21在胃癌组织中高表达(P<0.01),流式细胞仪检测发现,miR-21过表达对细胞凋亡和细胞周期影响不明显(P>0.05),miR-21过表达细胞株增殖能力增强,与阴性对照组和空白对照组对比差别具有统计学意义(P<0.01),miR-21转染细胞划痕愈合速度较对照组快。结论:miR-21在胃癌中呈高表达,过表达miR-21可以促进BGC-823胃癌细胞的增殖能力和迁移能力,但对凋亡和细胞周期影响不明显。Objective To investigate the expression of miR-21 in gastric cancer and its influence on biological behavior of gastric cancer cell. Methods The expression of rniR-21 in 30 resected gastric cancer and its surrounding normal tissue specimens and 10 gastric cancer cell lines was measured by real time fluorescence quantitative PCR. The influence of overexpression of miR-21 on cell proliferation, apoptosis, cell cycle and migration were evaluated by CCK-8 kit, Annexin-V ＋PI, flow cytometry （FCM）, and scratch healing assay. Results Real-time PCR showed overexpression of miR-21 in gastric cancer tissues （P〈0.01）. Over-expression of miR-21 had no obvious effect on apoptosis and cell cycle （P〉0.05）. Proliferation was enhanced in miR-21 overexpressed cell lines, significantly higher than that of negative controls and blank controls （P〈0.01）.Tbe healing rate of miR-21 transfected cells was faster then that of controls in scratch healing assay. Conclusions miR-21 is overexpressed in gastric cancer. Over-expression of miR-21 promotes cell proliferation and migration, but has no obvious effect on apoptosis and cell cycle.

关 键 词：胃癌:miR-21 增殖 迁移 

分 类 号：R735.2[医药卫生—肿瘤]