去氨微乳的结肠定向除氨作用  

Targeted removal of ammonia from the colon using ammonia-removing microemulsion

作　　者：王爱红[1,2] 段志军[1] 田舸[3] 张文君[4] 贺高红[4] 

机构地区：[1]大连医科大学附属第一医院消化内科,辽宁省大连市116011 [2]唐山市工人医院分院,河北省唐山市063000 [3]大连医科大学药学院,辽宁省大连市116041 [4]大连理工大学化工学院,辽宁省大连市116012

出　　处：《世界华人消化杂志》2009年第27期2821-2824,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30970886;大连市科技计划基金资助项目;No.2008E13SF193~~

摘　　要：目的:考察去氨微乳结肠定向除氨作用,期望为肝性脑病的防治提供一种更直接有效的新方法.方法:将去氨微乳放入含不同氨浓度的人工结肠液中10h,体外模拟胃肠转运时间与pH环境的实验;参照国际检测氨的方法分别测氨浓度变化,计算氨清除率;在氨检测装置中比较去氨微乳、空白微乳、水及乳果糖对氨的清除作用.结果:去氨微乳使含氨5g/L与10g/L的结肠液氨浓度均下降为0g/L,使含氨20g/L的结肠液氨浓度下降为0.521±0.135g/L(P<0.05).在含氨10g/L与20g/L的人工结肠液中对氨的清除率较在含酶的人工胃液与小肠液消化中显著提高(100%±0.00%vs96.41%±0.84%;97.29%±2.67%vs86.42%±2.63%,均P<0.05).去氨微乳清除氨的效果明显优于空白微乳、水及乳果糖.结论:去氨微乳本身具有很好的除氨作用,为清除肠道氨提供了新的方法,但其作用会受到胃与小肠消化的影响,需进一步优化其配方.AIM：To investigate whether ammonia-removing microemulsion （ARM） can specifically remove ammonia from artificial colonic fluid and explore the potential role of ARM in the prevention and treatment of hepatic encephalopathy. METHODS：ARM was mixed with artificial colonic fluid containing different concentrations of ammonia for ten hours. Meanwhile,ARM was successively mixed with artificial gastric juice for 2 h,artificial small intestinal juice for 3 h and artificial colonic fluid for 10 h to mimic gastrointestinal transit and pH environment. Ammonia concentrations in the fluid were then measured using a standard technique,and the ammonia removal rate was calculated. The ammonia removal efficiency achieved using ARM was compared with that achieved using empty microemulsion,water and lactulose. RESULTS：After mixing with ARM,the ammonia concentrations decreased to 0 g/L in the artificial colonic fluid initially containing 5 g/L or 10 g/L of ammonia,and to 0.521 ± 0.135 g/L in the fluid containing 20 g/L of ammonia. The ammonia removal rate achieved in artificial colonic fluid containing 10 or 20 g/L of ammonia was significantly higher than that in the artificial gastric juice and small intestinal juice containing enzymes （100% ± 0.00% vs 96.41% ± 0.84%,and 97.29% ± 2.67% vs 86.42% ± 2.63%,respectively; both P〈0.05）. ARM achieved a higher ammonia removal efficiency than empty microemulsion,water and lactulose. CONCLUSION：ARM shows a very good ammonia-removing effect and thus provides a new approach for removal of colonic ammonia. However,the digestive fluids in the stomach and small intestine may weaken such ammonia-removing effect.

关 键 词：肝性脑病 氨 微乳 

分 类 号：R747.9[医药卫生—神经病学与精神病学]