贵州省幽门螺杆菌临床菌株的分离培养  

Isolation and culture of clinical strains of Helicobacter pylori from patients in Guizhou Province

作　　者：胡林[1] 刘苓[1] 谭庆华[1] 周力[1] 陈峥宏[2] 刘娅琳[1] 

机构地区：[1]贵阳医学院附属医院消化内科,贵州省贵阳市550004 [2]贵阳医学院基础医学院,贵州省贵阳市550004

出　　处：《世界华人消化杂志》2009年第27期2830-2834,共5页World Chinese Journal of Digestology

基　　金：贵州省优秀科技教育人才省长专项资金资助项目;No.2008-94~~

摘　　要：目的:分离培养贵州省幽门螺杆菌(Helicobacter pylori,H pylori)的临床菌株,并探讨临床菌株成功分离培养患者的一般资料.方法:收集因上消化道症状就诊于我院行胃镜检查的患者98例,取胃窦黏膜经转运、接种、培养、鉴定、传代、增菌及分离培养H pylori.分析比较H pylori培养阳性患者在不同民族、取材部位、病种、性别及有无胃炎家族史等差异.结果:98例患者37例分离出H pylori临床菌株,阳性率38%.汉族标本培养阳性率为36%,少数民族标本为44%,2组无显著差异.其中苗族组培养阳性率为100%(4/4),与汉族组比较显著升高(P<0.05).慢性胃炎伴糜烂及消化性溃疡患者培养阳性率分别为39%(11/28)、46%(24/52),较单纯胃炎及胃癌患者显著增高(均P<0.05).30-60岁年龄段培养阳性率较60-80岁显著升高(45%vs21%,P<0.05).男性患者培养阳性率较女性显著提高(48%vs24%,P<0.05).有胃炎家族史患者培养阳性率与无胃炎家族史患者比较无显著差异.结论:本研究系我省首次成功分离出H pylori临床菌株,为贵州省H pylori基础及临床的研究奠定了基础.AIM：To isolate and culture the clinical strains of Helicobacter pylori （H pylori） from patients in Guizhou Province,and to determine the clinical features of patients from whom H pylori strains were isolated and cultured successfully. METHODS：Ninety-eight patients suffering from upper gastrointestinal symptoms underwent antra biopsy for isolation and culture of H pylori strains The ethnic group,disease,age,gender,and family history of gastritis or H pylori were compared among patients who were positive for H pylori.RESULTS：The total positive rate of H pylori was 38% （37/98）. No significant difference was noted in the positive rate of H pylori between Han ethnic patients 36% （29/80） and other minority ethnic patients （36% vs 44%,P〈0.05）. However,the positive rate of H pylori in Miao ethnic patients was significantly higher than that in Han ethnic patients （P〈0.05）. The positive rates of H pylori in patients with erosive gastritis （39%,11/28） and peptic ulcer （46%,24/52） were significantly higher than that in patients suffering from gastritis or gastric cancer （both P〈0.05）. The positive rate of H pylori was significantly higher in patients aged 30-60 years than in patients aged 60-80 years （45% vs 21%,P〈0.05）. The positive rate of H pylori was significantly higher in male patients than in female ones （48% vs 24%,P〈0.05）. No significant difference was noted in the positive rate of H pylori between patients with and without a family history of gastritis. CONCLUSION：This study represents the first successful isolation and culture of clinical strains of H pylori from patients in Guizhou province,which provides a basis for future basic and clinical research of H pylori infection in this area.

关 键 词：贵州省 幽门螺杆菌 分离培养 

分 类 号：R573[医药卫生—消化系统] R446.5[医药卫生—内科学]