细胞色素P450表氧化酶对人舌鳞癌细胞Tca-8113裸鼠皮下移植瘤生长的影响  

Effects of CYP epoxgenase on the growth of Tca-8113 human tongue squamous cell carcinoma cells xenografts of nude mice

作　　者：蒋建刚[1] 沈桂芬[1] 宁耀贵[1] 汪道文[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院内科,湖北武汉430030

出　　处：《现代肿瘤医学》2009年第11期2031-2036,共6页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30340067);科技部国际合作项目(编号:2005DFA30880)

摘　　要：目的:研究细胞色素表氧化酶对在体肿瘤生长的影响。方法:将转染rAAV-CYP2J2、rAAV-CYPF87V、rAAV-antiCYP2J2和rAAV-GFP的Tca-8113细胞,分别接种至裸鼠皮下,观察皮下移植瘤的生长情况,并用免疫组织化学染色分析移植瘤细胞PCNA和CD31分子的表达情况。结果:转染rAAV-CYP2J2、rAAV-CYPF87V明显促进裸鼠皮下移植瘤的生长,并且使移植瘤出现时间(分别为5.8天和6.0天)明显较对照组和转染rAAV-GFP(分别为8.4天和8.6天)短;而转染反义rAAV-CYP2J2组移植瘤生长缓慢,出现时间也明显延长(约9.75天)。免疫组化结果显示,rAAV-CYP2J2、、rAAV-CYPF87V明显促进移植瘤细胞PCNA和CD31的表达,移植瘤微血管密度明显增加。结论:CYP表氧化酶能明显促进在体肿瘤的生长,其机制可能与促进肿瘤血管生成密切相关。Objective：To study the effects of CYP epoxgenase on growth of primary tumors in nude mice. Methods： Tca - 8113 cells infected with rAAV - CYP2J2, rAAV - CYPF87V, rAAV - antiCYP2J2 and rAAV - GFP were inoculated into nude mice, respectively. The growth curve of epidermoid carcinoma xenograft was evaluated. The expression of PCNA and CD31 molecule were detected by using immunohistochemistry. Results：Xenograft in the rAAV - CYP2J2 and rAAV -CYPF87V infection groups （5.8 and 6 days after injection） displayed significantly earlier than that in the non - infection control group and rAAV - GFP infection groups （ 8.4 and 8.6 days after injection, respectively） and much earlier than that in the rAAV -antiCYP2J2 group （9.75 days after injection）. Immunohisto- chemistry analysis showed that infection of rAAV - CYP2J2, rAAV - CYPF87V significantly upregnlated the expression of PCNA and CD31 molecule and increased micro -vessel density in tumors and adjacent tumor interstitial tissues. Conclusion：These results provide a potent support to the conclusion that CYP epoxygenase is able to promote malignant proliferation of human cancers in vivo by increasing angiogenesis in tumors.

关 键 词：细胞色素P450表氧化酶 移植 增殖细胞核抗 微血管密度 

分 类 号：R730.3[医药卫生—肿瘤]