Gefitinib在肺癌细胞细胞膜色谱保留性的初步研究  

The initial investigation of gefitinib chromatographic retention on lung cancer cell membrane

作　　者：张佳[1] 王健生[1] 周苏娜[1] 莫非[1] 王嗣岑[2] 段小艺[1] 

机构地区：[1]西安交通大学医学院第一附属医院,陕西西安710061 [2]西安交通大学医学院,陕西西安710061

出　　处：《现代肿瘤医学》2009年第11期2042-2044,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30600744)

摘　　要：目的:探讨细胞膜色谱法应用于吉非替尼在肺癌细胞细胞膜上保留性。方法:应用人肺腺癌细胞A549制备肺癌细胞膜固定相,低压湿法装入色谱柱(10mm×2mm,ID)中,在37℃条件下,以5mmol/L磷酸钾缓冲液(pH7.4)为流动相,流速为0.2ml/min,在253nm检测波长下应用高效液相色谱检测吉非替尼在肺癌细胞膜的保留特性。结果:吉非替尼在肺癌细胞膜上有保留。结论:吉非替尼在肺癌细胞膜色谱柱中有保留,可进一步进行研究。Objective： To investigate Gefitinib chromatographic retention on lung cancer cell by cell membrane chromatography. Methods：A cell membrane stationary phase （CMSP） was prepared by immobilizing human pulmonary adenocarcinoma cell hne A549 on the surface of a silica carrier In this model, the column （ 10 mm×2 mm, I. D. ） packed with CMSP, 5 mmol/L potassium phosphate buffer solutions （ pH 7.4） as mobile phase with the flow rate of 0.2 mL/min at 37.5 ℃were used in the following studies. The retention characteristic of the Gefitinib was investigated under the chromatographic conditions above at the wave length of 253nm. Results： Gefitinib can retain on the lung cancer cell membrane. Conclusion： Gefitinib can retain on the lung cancer cell membrane chromatographic column, and can be further take into research.

关 键 词：吉非替尼 肺癌 细胞膜色谱 保留 

分 类 号：R734.2[医药卫生—肿瘤]