MAD2蛋白在人正常组织和胃癌组织中的表达  

The expression of MAD2 in human normal tissues and gastric carcinoma

作　　者：王莉[1] 尹芳[1] 杜煜蕾[1] 陈铮[1] 乔泰东[1] 吴开春[1] 樊代明[1] 

机构地区：[1]第四军医大学西京医院消化病院肿瘤生物国家重点实验室,陕西西安710032

出　　处：《现代肿瘤医学》2009年第11期2045-2048,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(C03031905)

摘　　要：目的:研究MAD2蛋白在人正常组织和胃癌中的表达及其临床意义。方法:用免疫组织化学方法检测23例人正常组织及102例胃癌及癌旁组织中MAD2蛋白的表达。结果:MAD2蛋白在23例正常组织中主要定位在胞核和胞浆中,在正常胃组织主要定位在胞浆,在胃癌组织中主要定位在胞核和胞浆。MAD2在胃癌中和癌旁组织的表达阳性率分别为70.59%(72/102)和38.24%(39/102),两者差异具有统计学意义(P<0.001)。MAD2蛋白表达与肿瘤组织分化程度呈负相关,与淋巴结转移呈正相关(P<0.001)。结论:MAD2蛋白在细胞有丝分裂监测点中发挥重要作用,其亚细胞定位在胃癌形成中发生变化,MAD2表达在胃癌发生过程中具有重要作用,对其检测有助于胃癌恶性程度评价和预后判断。Objective：To investigate the expression of MAD2 in human normal tissues and gastric carcinoma. Methods：Immunohistochemistry was performed to test 23 human normal tissues and 102 gastrict cancer specimens. Results：MAD2 was localized in both the nucleus and cytoplasm in normal tissues. In normal stomach, MAD2 was expressed mainly in cytoplasm, but it showed additional staining in the nucleus in majority of gastric cancers. The positive rates of MAD2 protein expression were 70.59% in gastric cancer tissue and 38.24% in normal gastric mucosa respectively. Significant difference of MAD2 protein expression was found between cancer tissue and normal mucosa（P 〈 0. 001 ） . MAD2 protein was in versely correlated with histological grade and lymph node metastasis（P 〈 0.001 ）. Condusion：MAD2 inside cells may play a role in mitotic checkpoint control and the subcellular localizations of this protein undergo a shift in the subcellular localization during malignant transformation. The change of MAD2 expression may be involved maily in gastric carcinogenesis and prognosis of gastric cancer.

关 键 词：有丝分裂纺锤体监测点 MAD2基因 人正常组织 免疫组织化学 染色体不稳定性 

分 类 号：R730.3[医药卫生—肿瘤]