NGX6基因联用5-Fu对人结肠癌细胞凋亡的影响  

Effect of NGX6 Gene with 5-Fu on the Apoptosis of Colon Cancer Cells

作　　者：连平[1] 郭勤[1] 彭娅[1] 肖志明[1] 刘芬[1] 王晓艳[1] 沈守荣[1] 李桂源[2] 

机构地区：[1]中南大学湘雅第三医院消化内科,长沙市410013 [2]中南大学湘雅医学院肿瘤研究所

出　　处：《中国肿瘤临床》2009年第21期1239-1242,1247,共5页Chinese Journal of Clinical Oncology

基　　金：国家重大科学研究计划(编号:2006CB910503);国家自然科学基金项目(编号:30370801,30770972,30500238);湖南省自然科学基金项目资助(编号:06JJZ0068)

摘　　要：目的:探讨侯选抑瘤基因NGX6联用5-Fu对结肠癌细胞凋亡的影响。方法:以稳定转染并表达NGX6基因的HT-29细胞与5-Fu联用作为实验组,以PDTC与5-Fu联用的HT-29细胞作为对照组,通过EMSA检测各组结肠癌HT-29细胞核转录因子-κB(NF-κB)的激活情况,利用MTT比色法检测各组细胞增殖的情况,吖啶橙(AO)/溴化乙啶(EB)双染法显微镜观测以及PI/Annexin-V双染流式细胞仪检测各组细胞凋亡情况。结果:稳定转染并表达NGX6基因的HT-29细胞以及应用了PDTC的HT-29细胞NF-κB的激活均明显受到抑制;与5-Fu作用的HT-29细胞组比较,5-Fu联合PDTC作用于HT-29细胞后,HT-29细胞增殖受到明显抑制,5-Fu诱导HT-29细胞凋亡作用增强;与5-Fu联合PDTC作用于HT-29细胞的对照组比较,在诱导细胞凋亡以及抑制细胞增殖方面,稳定转染并表达NGX6基因的HT-29细胞与5-Fu联用组和对照组所取得一致的效果,NGX6基因增强5-Fu对HT-29细胞增殖抑制的能力及诱导HT-29细胞凋亡的能力。结论:NGX6基因抑制了肿瘤细胞NF-κB的激活,具有增强5-Fu诱导结肠癌细胞凋亡的能力,其机制可能是抑制肿瘤细胞NF-κB的激活,NGX6基因对肿瘤的治疗及预后起积极作用。Objective： To evaluate the effect of NGX6 with 5-Fu on the apoptosis of colon cancer cells. Methods： The NGX6-transfected HT-29 cell line with 5-Fu was used in the test group. HT-29 cell line with 5-Fu and PDTC was used in the control group. The expression of NF-κB was detected by EMSA. The proliferation of HT-29 cell line was assayed by MTT. The effect of NGX6 on the apoptosis was detected by FCM. HT-29 cells were double-stained by PI/Annexin-V and AO/EB and observed by fluorescence microscopy. Results： The expression of NF-κB was inhibited in NGX6 transfected colon carcinoma cell group and in colon carcinoma cell group treated with PDTC. Treatment with the chemopreventive compounds 5-Fu and PDTC resulted in different responses in the effects of anti-proliferation and induced apoptosis of colon carcinoma cells. There was no significant difference in apoptosis between NGX6-transfected HT-29 cell line with 5-Fu and the cells in the control group. NGX6 gene enhanced the effect of 5-Fu on the proliferation and apoptosis of colon carcinoma cells. Conclusion： NGX6 gene can induce apoptosis and inhibit the proliferation of colon carcinoma cells. NGX6 gene can enhance the effect of 5-Fu on the proliferation and apoptosis of colon carcinoma cells through NF-κB pathway.

关 键 词：结肠癌 NGX6基因 核转录因子-ΚB 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]