应用不同材质容器对硝酸银染色效果的影响  

Application of Different Material Containers on Influence of Silver Nitrate Staining

作　　者：曲由[1] 聂坤[1] 于建春[1] 韩景献[1] 

机构地区：[1]天津中医药大学第一附属医院针灸研究所、分子生物学实验室,300193

出　　处：《天津医药》2009年第11期952-954,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30772839);教育部科学技术研究重点项目(项目编号:207006);教育部高等学校博士点专项科研基金项目(项目编号:20060063006);天津市应用基础及前沿技术研究计划重点项目(项目编号:07JCZDJC08800)

摘　　要：目的:比较使用不同材料的染色容器对蛋白质十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)银染显色速度及效果的影响。方法:以小鼠脑蛋白的SDS-PAGE为材料,凝胶分别在玻璃平皿、医用搪瓷盘和密胺塑料盘中进行银染,参照常规银染方法;同时采用一种优化的银染方法作为对照,在医用搪瓷盘中进行。银染后蛋白条带用Quantity One凝胶定量软件进行灰度值分析。结果:常规方法银染的凝胶,在玻璃平皿中显色最快,蛋白条带灰度值最高;在医用搪瓷盘中显色速度及灰度值均次之;在密胺塑料盘中显色速度及灰度值最低。对照优化方法银染的凝胶,显色速度和蛋白条带灰度值远高于常规银染的凝胶。结论:对于小鼠脑蛋白的SDS-PAGE银染,常规方法中,用玻璃平皿效果最好,医用搪瓷盘次之,密胺塑料盘最差。优化方法可以大大提高用医用搪瓷盘银染的效果。Objective：To compare the influences of application of different containers on developing speed and effectiveness of silver nitrate staining of protein in SDS-polyacrylamide gel. Methods：The mouse brain protein in SDS-PAGE was taken for material. The stained silver gels were carried out in accordance with routine silver staining method, in glass culture dish, enamel dish and melamine dish separately. Meanwhile an optimized silver staining method was used as control that was carried out in the enamel dish. After silver staining gray values of protein bands were analyzed by Quantity One gel analysis software. Results：The speed of developing the gels stained with routine method was the fastest. The gray values of protein bands were the highest in glass culture dish, secondary in enamel dish and the lowest in melamine dish. The developing speed and gray values of protein bands were much higher in control method of silver staining than that of routine method. Conclusion：In routine methods, the effectiveness of silver nitrate staining of protein in SDS-polyacrylamide gel was the best in glass culture dish, secondary in enamel dish and the lowest in melamine dish. The optimized silver staining method could improve the effectiveness using enamel dish.

关 键 词：银染色法 电泳 聚丙烯酰氨凝胶 免疫电泳 双向 玻璃 塑料 陶瓷制品 

分 类 号：R57[医药卫生—消化系统] R446[医药卫生—内科学]