稀土和硫酸二乙酯联合诱变米曲霉产氨基酰化酶的研究  

Study on Production of Aminoacylase by Aspergillus Oryzae by Compound Mutation with DES-Rare Earth

作　　者：刘丽华[1] 刘丽萍[1] 李文彬[1] 王霞[1] 

机构地区：[1]内蒙古工业大学化工学院,内蒙古呼和浩特010051

出　　处：《化学与生物工程》2009年第11期43-46,共4页Chemistry & Bioengineering

基　　金：内蒙古自然科学基金资助项目(200711020206)

摘　　要：为获得高产氨基酰化酶的米曲霉菌株,选用稀土硝酸镧和硫酸二乙酯对米曲霉菌株CICC-2339进行联合诱变,经摇瓶发酵筛选到氨基酰化酶高产菌株SDESLa,其酶活为39.63μmol.g-1.h-1,较出发菌株提高了39.50%,诱变效果显著。A high-yield aminoacylase strain SDESLa was obtained by compound mutation with DES-rare earth through fermentation screening method.Its enzyme activity was 39.63 μmol·g-1·h-1,which increased 39.50% than the start strain CICC-2339.The effect of mutation was obvious.

关 键 词：米曲霉 氨基酰化酶 联合诱变 

分 类 号：Q554.4[生物学—生物化学]