LRP16基因过表达对卵巢癌细胞HO8910生长及E-钙黏着素的影响  

Effects of overexpression of LRP16 on proliferation of ovarian cancer cells HO8910 and E-cadherin

作　　者：龙海红[1,2] 韩为东[1] 孟元光[2] 赵亚力[1] 伍志强[1] 田丽媛[1] 尹肖云[1,2] 陈美霞[1] 

机构地区：[1]中国人民解放军总医院基础医学所分子生物室,北京100853 [2]中国人民解放军总医院妇产科,北京100853

出　　处：《现代肿瘤医学》2009年第12期2250-2253,共4页Journal of Modern Oncology

基　　金：国家自然科学基金项目(No.30572096;No.30670809)

摘　　要：目的:探讨过表达肿瘤相关基因LRP16对卵巢癌细胞株HO8910生长的影响。方法:将含有LRP16基因真核表达载体pcDNA3.1-LRP16及空载体质粒转染卵巢上皮癌细胞株HO8910使其稳定过表达,用MTT法测各组细胞生长曲线,用Western blot方法检测E-钙黏着素(E-cadherin)蛋白的表达。结果:LRP16基因过表达对HO8910细胞增殖无影响,LRP16基因过表达的HO8910细胞的E-钙黏着素(E-cadherin)蛋白表达水平明显下降。结论:LRP16基因对卵巢上皮癌细胞HO8910的增殖无影响,但使E-钙黏着素(E-cadherin)表达明显下降。Objective：To investigate the effects of overexpression of LRP16 on proliferation of ovarian cancer cells （HO8910）. Methods： LRP16 eucaryotie expression vector（pcDNA3.1 -LRP16） and control vector were constructed and transfected into ovarian cancer cell lines （HO8910 cells）, in order to obtain stable overexpressing LRP16 cell lines and control lines ; The growth of each group was measured with MTT method ; The expression of E - cadherin pro- tein was determined by Western blot. Results： There was no effect of overexpression of LRP16 on the proliferation of HO8910 cells;The expression of E- cadherin significantly decreased in HO8910 cells as LRP16 overexpression. Conclusion： There was on effect of LRP16 on the proliferation of ovarian cancer ceils （HO8910 ） ;The expression of E - cadherin decreased in HO8910 cells as LRP16 overexpression. It was comfirmed that LRP16 improve the expression of E - cadherin in HO8910 cells .

关 键 词：LRP16基因 卵巢癌 上皮性 HO8910细胞 E-钙黏着素 

分 类 号：R730[医药卫生—肿瘤] R737.31[医药卫生—临床医学]