安体优Ⅰ抑制肿瘤淋巴管生成的实验研究  

The experiment research of inhibitory effect of ATU-Ⅰon lymphangiogenesis of HCa-F liver cancer in 615 mice

作　　者：高文仓[1] 杨平[1] 谢长生[2] 吴良村[2] 冯媛[3] 苗迎秋[3] 郑丛龙[3] 

机构地区：[1]大连大学附属新华医院,辽宁大连116021 [2]浙江省中医院,浙江杭州310000 [3]大连大学医学院,辽宁大连116000

出　　处：《现代肿瘤医学》2009年第12期2256-2259,共4页Journal of Modern Oncology

基　　金：辽宁省自然科学基金资助项目(编号:20070036)

摘　　要：目的:观察安体优I对615小鼠HCa-F肝癌淋巴道转移的影响,探讨其作用机理。方法:生理盐水、干扰素和安体优I分别作用于动物模型,3周后检测转移淋巴结计数及转移率;免疫组化检测VEGF-C和Flt-4,5′-Nase-ALP双重组织化学法检测淋巴管微密度。结果:模型组、α-IFN组和中药组转移淋巴结计数分别为5.88±2.30、4.13±1.64和3.50±1.91个;淋巴结转移率分别为78.6%、61.1%和57.4%;微淋巴管密度分别为25.25±4.59、22.38±4.41和21.75±4.71;VEGF-C的阳性率分别为87.5%、25.0%和25.0%,Flt-4阳性率分别为100.0%、37.5%和50.0%,中药组与模型组比较有显著性差异(P<0.05);结论:安体优Ⅰ具有抑制615小鼠HCa-F肝癌淋巴道转移作用,其机制可能与下调淋巴管生成信号通路VEGF-C及其受体Flt-4的表达,抑制肿瘤淋巴管生成相关。Objective： To observe the inhibitory effect of ATU - I on lymphatic metastasis of HCa - F liver cancer in 615 mice. Methods： To treat the mice model with NS,α -IFN and ATU - I ,count the number of metastasized lymph node and rate of metastasis 3 weeks later; detect the expression of VEGF - C and Fh - 4 with immunohisto- chemical method; detect the micro - lymph duct density （MLD） with 5＇ - Nase - ALP double staining method. Re- suits ： In Model group, α - IFN group and ATU - I group, the number of metastasized lymph node were 5.88 ＋ 2.30, 4.13 ± 1.64 and 3.50 ± 1.91, the rates of metastasis of lymph node were 78.6% ,61.1% and 57.4% ;MLD were 25.25±4.59,22.38±4.41 and 21.75±4.71 ,the positive rates of VEGF - C were 87.5% ,25.0% and 25.0% ,the positive rates of Fit -4 were 100.0% ,37.5% and 50.0% ,comparing with Model group,there was a significance different in ATU - I group（P 〈0.05）. Conclusion： There is the effect of inhibition to the metastasis of lymphdute of HCa - F liver cancer in 615 mice treated with ATU - I,and the mechanism relates with down regulate of VEGF - C/ Flt- 4 and inhibition of lymphangiogenesis of liver cancer.

关 键 词：中医药疗法 淋巴道转移 淋巴管生成 血管内皮生长因子-C 血管内皮生长因子受体-3 

分 类 号：R730[医药卫生—肿瘤] R735.7[医药卫生—临床医学]