纤维母细胞活化蛋白和转化生长因子β1在胃癌中的表达及其临床意义  

Expression of fibroblast activation protein and transforming growth factor β1 in gastric carcinoma and their implications

作　　者：王正彩[1] 王瑞芬[2] 刘杨[1] 李争艳[1] 王立峰[1] 

机构地区：[1]哈尔滨医科大学附属第一医临床医学院病理科,哈尔滨150001 [2]上海市第一人民医院宝山分院病理科,上海200940

出　　处：《诊断病理学杂志》2009年第5期368-371,共4页Chinese Journal of Diagnostic Pathology

基　　金：黑龙江省科技攻关计划项目(No.GB06C40203)

摘　　要：目的研究纤维母细胞活化蛋白(FAP)和转化生长因子β1(TGFβ1)在胃癌组织中的表达,并分析其与胃癌临床病理特征及淋巴结转移间的关系。方法采用免疫组化方法检测FAP和TGFβ1在42例胃癌和癌旁正常组织中的表达情况。结果①正常胃组织中FAP(-);胃癌组织中FAP主要表达于肿瘤相关纤维母细胞胞质中,且弥漫型胃癌表达显著高于肠型胃癌(P<0.05),并与临床病理分期、浸润深度和淋巴结转移呈正相关(P<0.05);②正常胃组织中TGFβ1为(-)或弱(+);胃癌组织中TGFβ1主要表达于瘤细胞胞质,在肿瘤间质纤维母细胞、血管内皮细胞和中性粒细胞偶有表达,且瘤细胞的表达强度与临床病理分期、浸润深度和淋巴结转移呈正相关(P<0.05);③FAP在肿瘤相关纤维母细胞的表达与TGFβ1在肿瘤细胞的表达呈正相关(P<0.05)。结论从FAP表达与TGFβ1表达的正相关性,推测其可能是通过肿瘤-间质相互作用促进肿瘤演进。FAP特异性表达于胃癌间质纤维母细胞,与多项临床病理指标,尤其是淋巴结转移和肿瘤的病理分型有关,可以作为预测胃癌淋巴结转移和预后的免疫病理学指标,有望成为抗胃癌治疗的一个新的靶向分子。Results （ 1 ） FAP was mainly expressed in tumor associated fibroblasts and the level of its expression in diffuse-type of gastric carcinoma was higher than that in intestinal-type （ P 〈 0.05） ; the expression was correlated with the TNM tumor stage, the depth of tumor invasion and lymph node metastases. （2） On the other hand, TGFβ1 mainly expressed in tumor cells and the level of its expression was correlated with TNM tumor stage, the depth of tumor invasion and lymph node metastases. （3） The expression of FAP in tumor associated fibroblasts was correlated with the expression of TGFβ1 in tumor cells. Conclusion FAP and TGFβ1 can serve as biomarkers for diagnosis and prediction of gastric carcinoma invasion and lymph node metastasis, as well as potential therapeutic targets in gastric carcinoma.

关 键 词：胃癌 肿瘤相关纤维母细胞 纤维母细胞活化蛋白 转化生长因子Β1 肿瘤-间质相互作用 

分 类 号：R735.2[医药卫生—肿瘤]