聚肌胞对小鼠前列腺癌内微血管的影响  

The influence of polyI:C on blood capillary in mouse prostate carcinoma

作　　者：田原僮[1] 曾昭毅[1] 陈伟伟[1] 何蔚[1] 赵丽娟[2] 

机构地区：[1]赣南医学院药理学教研室,江西赣州341000 [2]吉林大学白求恩医学院病理生理学教研室,吉林长春130021

出　　处：《中国药理学通报》2009年第11期1449-1452,共4页Chinese Pharmacological Bulletin

基　　金：科技部国际科技合作重点项目(No2004DFB02000)

摘　　要：目的探讨聚肌胞(polvriboinsine-polyribocyaidylic acid,polyI:C)对小鼠前列腺癌组织内血管生成的影响,初探其可能机制。方法将16只C57BL6/J纯系荷瘤小鼠按瘤重随机分为对照组和polyI:C组,分别瘤内注射生理盐水和polyI:C(每3d1次),用药7次后切除瘤灶。HE染色,镜下观察肿瘤组织组织形态学变化及血管分布情况。利用试剂盒检测肿瘤组织内NO水平。免疫组织化学染色,观察肿瘤组织eNOS、VEGF和AQP1蛋白表达情况。结果对照组和polyI:C组小鼠前列腺癌组织内的NO含量分别为(8.73±5.34)μmol和(1.22±0.77)μmol,两组比较差异有显著性(P<0.05)。免疫组化结果显示,对照组和polyI:C组的VEGF阳性染色前列腺癌细胞计数占同类肿瘤细胞的比例分别为(37.91±7.62)%和(9.64±2.90)%;C组的eNOS阳性染色前列腺癌细胞计数占同类肿瘤细胞的比例分别为(54.43±10.39)%和(22.00±8.07)%;AQP1平均值分别为(14.8±3.5)和(3.2±1.3),两组比较差异有显著性(P<0.05)。结论polyI:C可能通过下调小鼠前列腺癌组织中eNOS和VEGF蛋白的表达,抑制小鼠前列腺癌组织内血管的生成;通过影响组织内AQP1蛋白水平、减少NO生成和释放,影响血管内皮细胞的功能和肿瘤组织的微循环。Aim To investigate the effects of polyI：C on angiogenesis in mouse prostate carcinoma and its mechanisms.Methods Prostate carcinoma bearing mice were randomly divided into two groups according to tumor volume：contrl group and polyI：C group.After seven times′treatment,the mice were sacrificed.The content of NO in tumor was measured by nitric oxide assay kit.Tumor tissues were partly performed hematoxylin-eosin staining to observe morphological changes and distribution of vasa.Immunohisto chemical staining was used to observe the expression of VEGF,eNOS and AQP1.Results The content of NO in polyI：C group and the control was（1.22±0.77）μmol and（8.73±5.34）μmol respectively,and there was significant difference between two groups（P0.05）.The expression of VEGF in polyI：C group and the control was （9.64±2.90）% and（37.91±7.62）% respectively,and there was significant difference between two groups（P0.01）.The expression of eNOS in polyI：C group and the control was（22.00±8.07）% and （54.43±10.39）% respectively,and there was significant difference between two groups（P0.01）.The mean expression of AQP1 in polyI：C group and the control was（3.2±1.3） and （14.8±3.5） respectively,and there was significant difference between two groups（P0.05）.Conclusions Through downregulation the expression of VEGF and eNOS,polyI：C plays an important role in inhibiting angiogenesis of mouse prostate carcinoma.By reducing the expression of AQP1 and the content of NO,it influences endothelial cell function and local tumor microcirculation.

关 键 词：聚肌胞 小鼠 前列腺癌 VEGF ENOS NO AQP1 

分 类 号：R-332[医药卫生] R329.24