三氧化二砷体外抑制人星形细胞瘤细胞增殖及诱导凋亡的研究  

Inhibition of Proliferation and Induction of Apoptosis of a Glioma Cell Line by Arsenic Trioxide in vitro

作　　者：杨超[1] 夏之柏[1] 陈昆[1] 柯春龙[1] 黄正松[1] 

机构地区：[1]中山大学附属第一医院神经外科,广州510080

出　　处：《中国神经精神疾病杂志》2009年第11期677-679,共3页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金资助项目(编号:30770763)

摘　　要：目的探讨三氧化二砷(As2O3)对人脑胶质瘤细胞系LN444的增殖抑制和诱导凋亡的作用。方法用梯度浓度的As2O3处理LN444胶质瘤细胞,CCK-8比色法检测细胞的增殖活性,计算细胞增殖抑制率。Hoechst染色观察As2O3引起的核形态改变。流式细胞仪PI染色检测As2O3作用后的细胞凋亡率。结果As2O3对LN444胶质瘤细胞增殖有明显的抑制作用,各剂量组与空白对照组抑制率的差异有统计学意义(P<0.05),呈剂量依赖性和时间依赖性。Hoechst染色显示用药后细胞出现细胞凋亡的典型变化。PI染色显示As2O3处理后细胞凋亡显著增加,各剂量组间差异有统计学意义(P<0.05)。结论As2O3对人胶质瘤细胞具有增殖抑制和诱导凋亡的作用。Objective To study the effect of As2O3 an LN444 cell proliferation and apoptosis. Methods LN444 was treated with As2O3 at different concentrations. CCK-8 kit was used to measure cell viabilities after treatments, and then inhibition rates were calculated. Hoechst stain was applied to analyze the apoptotic effect. Flow cytometry with PI stain was used to detect the apoptosis rates after the treatments. Results As2O3 inhibited the LN444 cell proliferation significantly. The differences of inhibitory rates between the As2O3 treated groups and the control group were significant （P 〈 0. 05 ）. The inhibitory, effect was dose-and time-dependent. Nuclear shrinkage, irregular changes of nucleus shape, and nuclear fragmentation were observed in cells receiving As2O3. Treatment with As2O3 increased apoptosis in LN444 cells （ P 〈 0. 05 ）. Conclusions As2O3 significantly inhibits LN444 glioma cell proliferation and apoptosis in LN444 cells.

关 键 词：三氧化二砷 胶质瘤 增殖 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]