单一时间点HC2-HPV—DNA和HPVE6／E7 mRNA检测的临床研究  

Clinical research of HPV DNA detection and HPV RNA detection in single time

作　　者：卢文波[1] 姜智南[1] 陈顺美[1] 覃世榕[1] 赵玲军[1] 杨海涛[1] 施丹华[1] 陈雪梅[1] 

机构地区：[1]宁波市妇女儿童医院,浙江315012

出　　处：《中华实验和临床病毒学杂志》2009年第5期378-380,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：宁波市自然科学基金项目（2008A610087）

摘　　要：目的研究在单一时间点上的人乳头瘤病毒（HPV）E6／E7mRNA检测及HC2-HPV—DNA检测在宫颈疾病筛查中的应用。方法对2008年1月至2009年7月就诊的130例患者进行HPVE6／E7mRNA与HC2-HPV—DNA检测。以TCT结果为标准评价两种方法检测宫颈高度病变的效能。结果HC2-HPV-DNA检测阳性检出率82．3％（107／130），HPVE6／E7mRNA检测阳性检出率40．0％（52／130），两种方法在阳性检出方面差异有统计学意义（χ^2=24．5，P〈0．05）。HC2-HPV-DNA检测宫颈高度病变的敏感性为90．1％、特异性为22．1％、阳性预测值为37．4％、阴性预测值为82．6％；HPVE6／E7mRNA检测宫颈高度病变的敏感性为65．9％、特异性为73．3％、阳性预测值为55．8％、阴性预测值为80．8％。结论在单一时间点的宫颈癌筛查中结合HC2-HPV—DNA检测和HPVE6／E7mRNA检测更具有潜在价值。Objective To research the application of the single time detection of HPV E6/E7 mRNA and HC2-HPV-DNA in cervical screening project. Methods We detected both HPV E6/E7 mRNA and HC2-HPV- DNA of each sample which collected from 130 cervical disease patients＇ cervix during Jan. 2008 and July. 2009. TCT results were taken as standard to evaluate the diagnostic accuracy of the above two test methods in detecting high-grade cervical disease. Results 82.3%（107/130）women were confirmed to infect HPV by HC2-HPV-DNA detection, and 40.0% （52/130）women were confirmed to infect HPV by HPV E6/E7 mRNA detection, there was no significant difference between the two results（ χ^2 = 24.5, P 〈 0.05）. The sensitivity, specificity, positive predictive value, negative predictive value of HC2-HPV-DNA detection were 90.1%, 22.1%, 37.4% and 82.6 %, respectively. The sensitivity, specificity, positive predictive value, negative predictive value of HPV E6/ E7 mRNA detection were 65.9%, 73.3%, 55.8% and 80.8%, respectively. Conclusion In clinical cervical screening project of single time, the combination of HC2-HPV-DNA detection and HPV E6/E7 mRNA detection wick take on more potential value than applying each of them alone.

关 键 词：宫颈肿瘤 DNA RNA 乳头状病病毒 人 

分 类 号：R737.33[医药卫生—肿瘤] R373.9[医药卫生—临床医学]