乳腺癌组织中miR-21的表达及意义  

Expression of miR-21 in breast carcinoma and its significance

作　　者：于超[1] 吴诚义[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌外科,重庆400016

出　　处：《中国普通外科杂志》2009年第11期1126-1129,共4页China Journal of General Surgery

摘　　要：目的对乳腺癌组织及其相应癌旁组织中miR-21进行定量检测,分析其表达与临床病理特征的及意义。方法采用茎环实时荧光逆转录聚合酶链反应(RT-PCR)检测60例乳腺癌及其对应癌旁组织中miR-21的表达量,分析其表达与肿瘤大小、TNM分期、组织学类型、淋巴结转移、雌激素受体(ER)、孕激素受体(PR)等临床病理特征的关系。结果实时RT-PCR方法检测miR-21表达的敏感性和特异性良好。miR-21在乳腺癌组织中的表达明显高于癌旁组织(P<0.001);miR-21的高表达还与较高的TNM分期,淋巴结转移及PR的表达程度有关(各分组间P<0.01及<0.05);miR-21的表达在"三阴性乳腺癌"(ER,PR,CerbB-2均为阴性)和"非三阴性乳腺癌"之间的表达存在统计学差异(P=0.029)。结论miR-21在乳腺癌组织中高表达,其水平可能与乳腺癌的恶性程度有关。Objective To examine the level of expression of microRNA-21 （ miR-21 ） in breast cancer and its adjacent non-cancerous tissues, and analyze the miR-21 expression with the clinicopathological factors of breast cancer. Methods Specimens of breast cancer and adjacent tissues were collected from 60 patients. Their miR-21 expression was detected by Stem-loop real-time RT-PCR, and levels of miR-21 expression in tumor size, TNM clinical stage, histology, lymph node involvement, and hormone receptor status were compared. Results High sensitivity, specificity and precision of Stem-loop real-time RT-PCR allowed for direct analysis of expression of miR-21 in breast cancer tissues and adjacent tissues. The expression of miR-21 in breast cancer tissues was markedly higher than in adjancent tissue （ P 〈 0. 001 ）. Elevated miR-21 expression was also positively correlated with advanced TNM stage, lymph node metastasis, and low PR levels （P = 0. 007,0. 001, and 0.012 respectively）. Moreover, the levels of expression of miR-21 in triple-negative breast cancer（ TNBC ） were higher than those in non-TNBC types （ P = 0. 029 ）. Conclusions High levels of expression of miR-21 are correlated with degree of malignancy of breast cancer.

关 键 词：乳腺肿瘤 三阴乳腺癌 MICRORNA MIR-21 荧光定量PCR 

分 类 号：R737.9[医药卫生—肿瘤]