乳腺癌裸鼠骨转移模型的建立及评估  

Construction and evaluation of bone metastasis of breast cancer in nude mice model

作　　者：王江涛[1] 王捷[1] 欧陕兴[2] 乔国庆[2] 唐咸国[1] 李小荣[2] 蔡俊杰[2] 

机构地区：[1]广州军区广州总医院医学实验科,广东广州510010 [2]广州军区广州总医院放射线科,广东广州510010

出　　处：《中国普通外科杂志》2009年第11期1156-1160,共5页China Journal of General Surgery

基　　金：广东省自然科学基金研究团队项目(20023001)

摘　　要：目的建立乳腺癌骨转移动物模型并探讨对该转移模型的最佳评估方法。方法将带GFP标记的人乳腺癌细胞MDA-MB-231注入裸鼠左心室,建立乳腺癌骨转移模型。6周后使用X线机、活体显像系统和病理学检查对裸鼠骨组织进行评估。结果6周后病理学检查肿瘤细胞已扩散至裸鼠的脑、骨髓腔、肺等组织,X线影像清晰显示骨质破坏的位置及程度,活体显像系统可以观察到肿瘤细胞在裸鼠体内的分布。结论X线、活体显像和组织学检查3种方法相结合是分析乳腺癌小鼠骨转移模型的最佳方法之一。Objective To establish nude mice model of bone metastasis of breast cancer, and study the best method to evaluate the model. Methods The human breast cancer cell, MDA-MB-231PEGFPN, was injected into left ventrieule of nude mice to construct the bone metastasis of breast cancer animal model. Methods of X-ray, whole body optical imaging and pathology were used to evaluate the bone damage after six weeks. Results After six weeks, the result of pathology showed that breast cancer cells had already spread to brain, bone marrow and lung of nude mice. The degree of bone destruction was seen clearly in X-ray, and location of breast cancer cells was observed by whole body optical imaging system. Conclusions The results showed that combined application of X-ray, whole body optical imaging and pathology are the best modalities to evaluate bone metastasis of breast cancer in nude mice.

关 键 词：乳腺肿瘤 骨转移 疾病模型 动物 X线 活体显像 裸鼠 

分 类 号：R737.9[医药卫生—肿瘤]