成人成肌细胞的分离与体外培养  

Isolation and in vitro culture of human myoblasts

作　　者：陈勇[1] 周建大[2] 李文[2] 邹永华[1] 殷照初[1] 陈继业[2] 李明[2] 

机构地区：[1]湖南省计划生育研究所,湖南长沙410008 [2]中南大学湘雅三医院整形烧伤外科

出　　处：《中国医师杂志》2009年第11期1462-1465,共4页Journal of Chinese Physician

基　　金：湖南省自然科学基金重点项目（07JJ3067）;湖南省科学技术项目（2008SK3114）;湖南省卫生厅科研项目（B2007086）;中南大学创新基金项目（200807）

摘　　要：目的建立一种优化的体外成人成肌细胞分离与原代培养方法。方法首先用两步消化法对取自成人骨骼肌组织进行消化获取相对较纯的成肌细胞，通过差速贴壁法进行进一步的纯化，并对获取的成肌细胞进行细胞形态学观察、免疫组织化学、透射电镜鉴定及生长曲线的绘制与分析。结果该方法可获取高纯度的成肌细胞，且在体外可快速稳定增殖。结论该方法所获取的成肌细胞可为Duchenne肌营养不良的治疗、血友病、血栓闭塞性脉管炎（伯格氏病）、心肌梗死和慢性心衰的基因治疗的研究提供一种充足、可靠的实验靶细胞。Objective To establish an optimal method for in vitro culture and amplification of myoblasts from human skeletal muscle. Methods The myoblasts were isolated from human skeletal muscle with two steps combined dissociation, then the harvested myoblasts were purified by the way of different attachment. The cultured ceils were identified by morphology, immunohistochemistry and transmission electron microscopy. Growth curves were plotted and analyzed. Results A lot of highly pure myoblasts could be obtained by this procedure and cultured in vitro steadily and rapidly. Concision It shows promise for the treatment of DMD, hemophilia, Buerger＇s disease, myocardial infarction and chronic heart failure.

关 键 词：成肌细胞 骨骼肌 细胞分离 细胞培养技术 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]