双向凝胶电泳筛选子宫内膜癌淋巴转移相关蛋白质的研究  

Identification of the lymph node metastasis associated proteins in endometrial carcinoma by two dimensional gel electrophoresis.

作　　者：张进[1] 席晓薇[1] 张佳荣[1] 蔡斌[1] 杨懿霞[1] 万小平[1] 

机构地区：[1]上海交通大学附属第一人民医院妇产科,上海200080

出　　处：《现代妇产科进展》2009年第10期726-730,共5页Progress in Obstetrics and Gynecology

基　　金：上海市卫生局科研基金资助项目(No:044023)

摘　　要：目的:研究与子宫内膜癌淋巴转移相关的蛋白质。方法:提取正常子宫内膜、淋巴结阴性和淋巴结阳性的子宫内膜癌组织总蛋白,用双向凝胶电泳分离蛋白质,用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)分析鉴定差异蛋白点。结果:在淋巴结阴性和淋巴结阳性的子宫内膜癌中,共筛选出11个差异蛋白,与淋巴结阴性组比较,淋巴结阳性内膜癌中,热休克蛋白27、3种肌动蛋白、S100钙结合蛋白A9、丙酮酸激酶、α烯醇化酶、白蛋白和组氨酸三聚体核苷结合蛋白1表达上调,天冬酰胺酶样1蛋白和酯酶D/甲酰-谷胱甘肽水解酶下调。结论:淋巴结阳性和阴性子宫内膜癌的双向凝胶电泳图谱之间存在较明显差异。经鉴定的9种蛋白质可能在子宫内膜癌发生淋巴转移过程中发挥作用。Objective：To investigate the lymphatic node metastasis （LNM） associated protein in endometrial carcinoma（EC）.Methods ：Tissues from normal endometria,LNM（-） EC and LNM （＋） EC were unalyzed by two-dimensional gel electrophoresis.The differently expressed proteins were identified by matrix-assisted laser desorption of flight mass spectrometry （MALDI-TOF-MS）. Results： Eleven proteins were found to be differentially expressed between LNM（-） EC and LNM（＋） EC. Eaeh of them was identified. Among them,the heat shock protein 27, actin, S100 calcium-binding protein A9,pyruvate kinase, alpha enolase, serum albumin,histidine triad nucleotide binding protein 1 were up-regulated,while the ASRGL 1 protein and esterase D/formylglatathione hydrolase down-regulated compared with that in the LNM（-） EC. Conclusion：Significant difference of proteins express is found in the LNM（-） EC and LNM（＋） EC by two-dimensional gel electrophoresis. Nine proteins identified may play a role in the process of lymph node metastasis of EC.

关 键 词：双向凝胶电泳 基质辅助激光解吸电离飞行时间质谱 子宫内膜癌 淋巴转移 

分 类 号：R737.33[医药卫生—肿瘤]