氧化应激在复合致病因素法诱导大鼠肝硬化中的作用  

Effect of Oxidative Stress in the Development of Liver Cirrhosis Induced by Multiple Pathogenic Factors in Rat

作　　者：冀菁荃[1] 王来好[2] 贾建桃[1] 张丽丽[1] 田小霞[1] 王黎敏[3] 武延隽[4] 张慧英[1] 

机构地区：[1]长治医学院病理生理学教研室,046000 [2]长治医学院附属和平医院核医学科 [3]长治医学院机能实验室 [4]长治医学院病原实验室

出　　处：《长治医学院学报》2009年第5期321-324,共4页Journal of Changzhi Medical College

基　　金：山西省归国留学人员资助项目(200810)

摘　　要：目的:探讨肠源性内毒素血症(IETM)、高同型半胱氨酸血症以及氧化应激在复合致病因素法诱导的大鼠肝硬化形成过程中的作用。方法:将61只大鼠随机分为正常对照组、肝硬化模型2周组、4周组、6周组及8周组。摘取肝脏称重,计算肝系数;腹主动脉取血,测定血浆内毒素、同型半胱氨酸(HCY)、丙氨酸氨基转移酶(ALT)和总胆红素(TBil)水平;测定肝组织匀浆中肿瘤坏死因子(TNF-α),Ⅲ型前胶原(PⅢP)和总抗氧化能力(T-AOC)水平。结果:模型各组血浆内毒素、ALT活力和TBiL水平均高于对照组(P<0.05);同型半胱氨酸(HCY)随病程进展逐渐升高(P<0.05)。模型各组肝组织匀浆TNF-α、PⅢP水平明显升高,T-AOC明显降低(P<0.05)。结论:肠源性内毒素血症(IETM)、高同型半胱氨酸血症、氧化/抗氧化失平衡是肝硬化发生的重要机制。Objective：To explore the effects of intestinal endotoxemia（IETM） ,hyperhomocystinemia,and oxidation stress in the development of liver cirrhosis induced by multiple pathogenic factors in rats. Methods： Sixty - one male Wistar rats were randomly divided into five groups： normal control group, 2w, 4w, 6w, and 8w liver cirrhosis groups. Endotoxin, homocystine （HCY）, ALT, and TBil in plasma,and TNF- α, PIIIP and T- AOC in liver tissue were detected. Results：The plasmatic endotoxin, HCY, ALT, and TBil,and the TNF- α and ProP of liver tissue were significantly increased in all model groups,and the T- AOC of liver tissue were markedly decreased compared with normal control group （ P 〈 0.05）. Conclusion：The oxidative stress enlightened by ITEM and hyperhomocystinemia may be involved in the pathogenesis of fibrosis in hepatic cirrhosis induced by multiple pathogenic factors.

关 键 词：肝硬化 内毒素 氧化应激 同型半胱氨酸 

分 类 号：R363[医药卫生—病理学]