人晶状体上皮细胞体外培养  

Human lens epithelial cell cultrured in vitro

作　　者：雷在枝[1] 冯雪亮[2] 李冰[3] 

机构地区：[1]山西医科大学第一临床医学院眼科,太原030001 [2]山西省眼科医院斜视与小儿眼科 [3]山西省眼科医院角膜病科

出　　处：《山西医科大学学报》2009年第11期992-994,共3页Journal of Shanxi Medical University

基　　金：山西省出国留学回国人员基金资助项目(200288)

摘　　要：目的建立一种简单有效的人晶状体上皮细胞体外培养的方法。方法用组织块培养法,对不同年龄组(儿童组16例,中青年组18例,老年组14例)的人晶状体前囊膜进行培养。对培养的细胞进行形态学和增殖活动观察。结果组织块培养法较易在体外培养人类晶状体上皮细胞并传代,其增殖能力与供体年龄有关,但是老年人晶状体上皮细胞无法传代。结论成功地建立起人晶状体上皮细胞体外培养模型,可用于白内障及后发性白内障时后囊膜混浊发病机制和药物试验研究。Objective To establish a simple and effective procedure for culturing human lens epithelial cells（HLEC） in vitro. Methods The specimens of human lens capsule from the children, the young middle age and the aged were cultured using tissue explant method. The morphology and proliferation activities of cultured ceils were observed. Results HLECs were easily cultured and passaged in vitro using this procedure and the proliferative ability was negatively correlated to the age of donors. HLECs could not be passaged in the aged. Conclusion The method is successful and easy to handle, and may be utilized in the study on pathogenesis and pharmacology of cataract and posterior capsular opacification.

关 键 词：晶状体 上皮细胞 细胞培养 

分 类 号：R776.1[医药卫生—眼科]