左卡尼汀对四氯化碳致动物肝损伤的保护作用  

THE PROTECTIVE EFFECT AND MECHANISM OF L-CARNITINE ON ACUTE EXPERIMENTAL HEPATIC INJURY MICE AND RATS INDUCED BY CCL_4

作　　者：邵士川[1,2] 梁孝印[1,2] 刘叶玲[1,2] 冀承锋[1] 李宏建[3] 王春波[1] 

机构地区：[1]青岛大学医学院,青岛266071 [2]泰安市中心医院 [3]山东千佛山医院

出　　处：《现代预防医学》2009年第22期4320-4323,4326,共5页Modern Preventive Medicine

摘　　要：[目的]研究左卡尼汀(L-carnitine,LC)对小鼠和大鼠实验性肝损伤的保护作用和机制。[方法]复制四氯化碳(CCl4)所致急性和慢性肝损伤的动物模型(小鼠腹腔注射CCl4,大鼠皮下注射CCl4)。将受试动物随机分为正常对照组,CCl4模型组,LC组,硫普罗宁片(tiopronin tablets)对照组或水飞蓟片(legalon tablets)组。用酶生化法测定血清生化指标谷丙转氨酶(ALT)和谷草转氨酶(AST)的水平,总胆红素(T-BIL)、总蛋白(TP)、白蛋白(ALB)、白球比值(A/G),肝组织超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)和抗超氧阴离子能力(A-ASC)的活性、总抗氧化能力(T-AOC)以及脂质过氧化产物丙二醛(MDA)的含量。同时计算肝系数(liverindex,LI),行慢性肝损伤组织病理学检查。[结果]经口给予各剂量LC(0.25-1.0g·kg-1)后急性肝损伤动物血清ALT、AST及LI均明显降低,呈现剂量依赖性(P﹤0.05,P﹤0.01),大鼠和小鼠肝组织内SOD、GSH-px、A-ASC的活性及T-AOC水平均升高(P﹤0.05,P﹤0.01),而MDA的含量随LC剂量的升高而降低(P﹤0.05,P﹤0.01)。0.15g·kg-1的LC能显著降低慢性肝损伤大鼠血清ALT、AST及LI、T-BIL(P﹤0.01),增加TP、ALB和A/G比值(P﹤0.01),并有效减轻大鼠肝组织病理性改变。[结论]LC对CCl4所致的动物急慢性肝损伤均具有保护作用,其机制与其抗氧化特性有关。[Objective] To investigate the protective effect and possible mechanism of l-carnitine on experimental liver injury of mice and rats induced by CCl4.[Methods] We duplicated the acute and chronic liver injury models with CCl4 by intraperitoneally and subcutaneouly injecting CCl4 for mice and rats respectively.Mice and rats were divided into different groups randomly：normal control groups,model control groups,drug control groups and l-carnitine groups.The level of serum ALT,AST,T-BIL,TP,ALB,A /G and the activity of SOD,MDA,GSH-px,A-SAC and T-AOC in injury liver were detected by automatic biochemistry analyzer.The liver was weighted and the index of liver was calculated.[Results] After treating with different level of LC（0.25-1.0 g·kg-1）,the level of serum ALT,AST and liver index obviously decreased（P﹤0.05,P﹤0.01）,showed dose-dependent relationship.The activities of SOD,A-ASC and GSH-px（P﹤ 0.05,P﹤ 0.01） was obviously increased.The potential of T-AOC（P﹤0.05,P﹤0.01） was enhanced,reduce the content of MDA（P﹤0.05,P﹤0.01） increased with the decrease of LC（P﹤0.05,P﹤0.01）.LC（0.15g·kg-1） could significantly decrease the level of ALT,AST,LI,TP and T-BIL,and incrased the ALB and A /G（P﹤0.01） on chronic hepatic injury in rats,as well as relieve histopathological change of hepatic injury in rats.[Conclusion] LC has a remarkably protective effect on acute and chronic liver injury,and its mechanism has association with its antioxidation.

关 键 词：左卡尼汀 四氯化碳 急(慢)性肝损伤 大鼠 小鼠 

分 类 号：R575.1[医药卫生—消化系统]