气相色谱法分析沙门氏菌脂肪酸组成的研究  

STUDY ON THE CELLULAR FATTY ACIDS COMPOSITION OF SALMONELLA BY UNQUALIFICATION RATES ANALYSIS

作　　者：宋红梅[1] 李波[1] 李丽婕[1] 曹春红[1] 田润[2] 张雅薇[2] 

机构地区：[1]石家庄市疾病预防控制中心,石家庄050016 [2]石家庄市卫生监督局

出　　处：《现代预防医学》2009年第22期4324-4326,共3页Modern Preventive Medicine

摘　　要：[目的]获得沙门氏菌特征脂肪酸组成本底资料,评价脂肪酸分析方法在沙门氏菌属及型水平的判断能力。[方法]选取不同年代、不同血清型的沙门氏菌分离株117株,提取脂肪酸,应用SherlockMIS系统进行菌体脂肪酸组成及含量的分析。[结果]所有菌株共有的脂肪酸成分有12种,通过结合菌落特征,沙门氏菌属水平的判断符合率达到92.3%。[结论]MIDI菌库中沙门氏菌脂肪酸数据不完善,可通过与传统形态、生化反应结合方式实现快速鉴定。本试验同时提供了我国沙门氏菌脂肪酸组成基础数据。[Objective] To collect CFAs（cellular fatty acids） background data of Salmonella in China,and to evaluate the application value of fatty acid methyl ester（FAME） method in Salmonella strain identification.[Methods] 117 Salmonella strains belonging to 8 serotypes were isolated from 2005 to 2007 in China,and selected and fatty acid methyl ester were extracted.Sherlock Microbial identification system was used to analyze the data.[Results] Twelve fatty acids were the common components contained in all of the tested strains.Combining with the colonial morphology,the coincidence of generic judgment was 92.3%.[Conclusion] FAME method is useful in rapid identification of Salmonella strains.But the CFAs information of Salmonella in Sherlock standard library is deficient.A CFAs database for Chinese isolates of Salmonella has been developed.

关 键 词：沙门氏菌 气相色谱 脂肪酸 

分 类 号：R378.22[医药卫生—病原生物学]