3,4-亚甲基二氧基甲基苯丙胺诱导大鼠神经元凋亡及凋亡相关因子的表达  

Neuron Apoptosis Induced by 3,4-methylenedioxy Methamphetamine and Expression of Apoptosis-related Factors in Rat Brain

作　　者：王雪[1] 祝三平[2] 况伟宏[1] 李静[1] 孙啸[3] 黄明生[1] 孙学礼[1] 

机构地区：[1]四川大学华西医院精神科,成都610041 [2]四川大学研究生院 [3]四川大学华西临床医学院临床医学专业05级

出　　处：《四川大学学报（医学版）》2009年第6期1000-1002,1037,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川大学华西医院基金(基金编号:134040162)资助

摘　　要：目的研究腹腔注射(ip)3,4-亚甲基二氧基甲基苯丙胺(MDMA)对实验大鼠大脑神经元的凋亡诱导作用,以及凋亡相关因子Caspase-3和细胞色表C(Cyt C)在不同脑区的表达情况。方法将20只大鼠均分为4组,随机选3组为MDMA实验组(B、C、D),另1组为对照组(A)。B组予MDMA 20 mg/kg,ip,单次,C组予MDMA20 mg/kg Bid×2 d,ip,D组予MDMA20 mg/kg Bid×4 d,ip;A组给予等体积生理盐水(ip,单次)。采用TUNEL法检测神经元的凋亡,免疫组织化学方法检测Caspase-3和Cyt C的表达。结果与对照组相比,给予MDMA后,B、C和D组大鼠各相关脑区(额叶皮层、海马和纹状体)有凋亡细胞形成,Caspase-3和Cyt C有程度不同的表达。C组和D组较B组的凋亡细胞增加且凋亡相关因子的表达增加(P<0.05)。结论MDMA可导致实验大鼠神经元的凋亡,并诱导凋亡相关因子Caspase-3和Cyt C的表达。Objective To study the neuron apoptosis induced by i.p 3,4-methylenedioxy methamphetamine（MDMA） and the expression of apoptosis-related factors in rat brain.Methods Twenty rats were divided into 4 groups.In group A,the rats were injected intraperitoneally with single dosage of saline,while the rats of group B,C,D were injected i.p with MDMA in different regimen,which were 20 mg/kg,single injection in group B,20 mg/kg twice a day（8 am and 8 pm）,for 2 day in group C,as well as 20 mg/kg,twice a day（8 am and 8 pm） for 4 days.Neuron apoptosis were measured by TUNEL,and the expression of Caspase-3 and CytC were detected by immunohistochemistry. Results Compared with saline group,apoptosis neurons were detected at the related brain regions（such as frontal cortex,hippocampus and striatum） of the rats in MDMA treated groups;Expression of Caspase-3 and CytC was observed at different level.Compared with group B,the number of apoptosis neurons of group C and D increased,and also the apoptosis-related factors in the brain tissue increased（P〈0.05）.Conclusion MDMA could induce neurons apoptosis and the expression of apoptosis-related factors such as Caspase-3 and CytC in rat brain.

关 键 词：3 4-亚甲基二氧基甲基苯丙胺 神经元凋亡 CASPASE-3 CYT C 

分 类 号：R114[医药卫生—卫生毒理学]