靶向超声造影剂促进反义基因转染的体外研究  

Enhanced Gene Transfection in Vitro with Targeted Ultrasound Contrast Agent

作　　者：程文[1] 郭文佳[1] 荆慧[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院超声科,哈尔滨市150040

出　　处：《中国超声医学杂志》2009年第11期1021-1024,共4页Chinese Journal of Ultrasound in Medicine

基　　金：黑龙江省自然科学基金(No.D200414);黑龙江省教育厅科学技术研究项目(No.10541129)

摘　　要：目的探讨超声破坏造影剂微泡联合半乳糖化多聚赖氨酸(G-PLL)对基因治疗肝癌的靶向促进作用。方法将合成的超声造影剂微泡、G-PLL及c-myc反义寡核苷酸(ASODN)耦联物,结合超声辐照作用于表达c-myc基因的人肝癌及人肺癌细胞株进行体外效应研究。观察细胞形态学改变及耦合物与细胞结合情况并检测c-myc基因的表达情况。结果镜下显示肝癌细胞较肺癌细胞更易于与耦联物结合,多数细胞呈凋亡改变。经超声辐照,耦联物作用后的人肝癌及肺癌细胞c-myc基因表达量均有降低,而G-PLL对肝癌细胞c-myc基因表达有明显抑制作用。结论超声辐照下,造影剂结合G-PLL及反义基因耦联物可靶向性促进反义基因转染肝癌细胞。Objective To investigate the improved effects of ultrasound irradiation microbubble agent conjugating galactose-terminal glyco-poly-1-lysine （G-PLL） and antisense oligodeoxynucleotides （ASODN） in the treatment of hepatoma. Methods We established SMMC-7721 hepatoma cells model and A-549 pulmonary carcinoma cells model both of which had c-myc gone expression. G-PLL was bound to microbubble and c-myc ASODN. Different compounds were given to respective experimental groups and soon were treated with ultrasound probe irradiation. We observed every group under powerful microscope every day. C-myc mRNA was detected by RT-PCR after 48 hours. Results After 48 hours transfection and observation under fluorescence microscope, the SMMC-7721 cell had conside table fluorescent material on the surface of the cells. Between experimental groups, SMMC-7721 cells group had a significant difference, but there was no significant difference in A549 group. Conclusions Ultrasound irradiation, the compounds of microbubble agent, G-PLL and ASODN have targeted inhibitive effect on the c-myc gene expression of SMMC-7721 hepatoma cells.

关 键 词：超声造影剂 超声照射 肝靶向 基因治疗 

分 类 号：R735.7[医药卫生—肿瘤]