TFPI-2对K562细胞体外生长和侵袭能力的影响  

The Influence of TFPI-2 on the Growth and the Invasion of K562 Cells in vitro

作　　者：许云[1,2] 杜新[2] 张蕴芳[2] 陶小梅[2] 楼瑾[2] 黄瑞宏[2] 

机构地区：[1]广州医学院,2006级硕士研究生510182 [2]深圳市第二人民医院血液病研究所,518035

出　　处：《医学研究杂志》2009年第9期46-49,F0003,共5页Journal of Medical Research

基　　金：广东省自然科学基金(04007151)

摘　　要：目的研究组织因子途径抑制物-2(TFPI-2)基因对白血病细胞系K562细胞生长和侵袭的影响。方法通过脂质体法将TFPI-2基因转染到K562细胞,用实时定量RT-PCR和Western blot分别检测转染前后TFPI-2mRNA和其蛋白质的表达。生长曲线测定,平板克隆和(Transwell)小室穿透实验测定转染前后3组细胞生长、恶性增生能力以及体外侵袭能力的差异。结果转染成功TFPI-2基因的K562细胞(K562-T)有TFPI-2mRNA及其蛋白质的表达,与对照组K562组、K562-V组细胞相比,K562-T细胞生长速度缓慢,克隆形成率明显低于前2组(P<0.05),有统计学意义;其穿膜细胞数(16±4.51)明显低于前两者(33.67±4.51)及(28.67±3.51),P<0.05,有统计学意义。结论TFPI-2基因对K562细胞的生长和侵袭能力有抑制作用。Objective To evaluate the influence of tissue factor pathway inhibitor 2 （TFPI - 2） gene on the proliferation and invasion of K562. Methods The expression vector pcDNA3.0/TFPI -2 was transfected into human leukemia line K562 cells（ K562 -T）by using liposome, then the mRNA and protein TFPI - 2 were detected by real - time RT - PCR and western blot separately. The growth curve and the colony - forming unit assay were used to measure the ability of cells growth and transwell chamber model was employed to test the ability of cell invasion in vitro. Results Expression of mRNA and protein of TFPI - 2 was detected in transfected cells. The growth rate and self - replication ability of K562 - T cells were lower than those of the two control groups obviously. The number of K562 - T cells to traverse a Matrigel - coated membrane was dramatically decreased compared with that of non - expressing cells. Conclusion The gene of TFPI - 2 can inhibit the growth, proliferation and invasion of the K562 cells.

关 键 词：组织因子途径抑制物-2 K562细胞 增生 侵袭 

分 类 号：R733.7[医药卫生—肿瘤] R735.9[医药卫生—临床医学]