CPT-11在鼻咽癌裸鼠组织中时辰给药疗效观察及细胞周期分布变化的初步研究  

The Efficacy of CPT-11 Temporal Dosage for Nasopharyngeal Carcinoma in Nude Mice and Its Effect on Cell Cycle Distribution

作　　者：范娟[1] 韩云炜[1] 陈枫[1] 吴敬波[1] 汪碧琼[1] 

机构地区：[1]泸州医学院附属医院肿瘤科,四川省泸州市646000

出　　处：《中国肿瘤临床》2009年第20期1190-1193,共4页Chinese Journal of Clinical Oncology

基　　金：泸州市科学枝术局科研项目基金资助(编号:泸市科[2009]72号)

摘　　要：目的:观察CPT-11(艾力)时辰给药对鼻咽癌裸鼠的疗效、毒副反应以及细胞周期分布的变化,为CPT-11时辰化疗提供实验基础和临床应用参考。方法:统一光照条件下(光照时间7:00～19:00),黑暗时间(19:00～7:00)饲养3周、50只鼻咽癌移植瘤裸鼠模型分为给药组和对照组。给药组荷瘤小鼠在3、9、15、21HALO(Hours After Light Onset设光后小时)腹腔注射CPT-11 66mg/kg;15天后处死所有小鼠,检测各组小鼠的WBC、HB、PLT,然后剥离肿瘤计算各组抑瘤率,制成单个细胞悬液,固定,染色后流式细胞仪测DNA细胞周期含量;并取盲肠组织光镜下观察肠粘膜组织结构。结果:CPT-11对鼻咽癌移植瘤裸鼠有显著性疗效(P<0.01),其中15HALO,抑瘤率最大(P<0.05);而21HALO抑瘤率最小(P<0.05)。15HALO给药组WBC、HB、PLT数均高于其他各组(P<0.05),体重增加明显(P<0.05),光镜下粘膜损伤分级显著降低(P<0.05),21HALO给药组血象、体重增加最少,粘膜损伤分级较高(P<0.05)。9HALO要优于3HALO(P<0.05)。21HALO,3HALO细胞主要分布在G_2期(54.1100±17.3374053.0300±20.79316)。其中15HALO与21HALO差异最明显(P<0.01)。结论:CPT-11对鼻咽癌移植瘤裸鼠有明显的抑制作用且疗效及毒副作用呈现时辰节律变化(15HALO最优,21HALO最差),为CPT-11时辰化疗的临床应用提供了参考。Objective： To observe the curative effect and toxic and side effects of CPT-11 （Irinotecan） chrono-chemotherapy on nasopharyngeal carcinoma in nude mice and the effect of such treatment on the cell cycle distribution in nasopharyngeal carcinoma cells. Methods： Fifty nude mice with nasopharyngeal carcinoma were maintained for 3 weeks on a normal chow diet and housed in a room with a 12：12-h light-dark cycle and an ambient temperature of 22%. These mice were divided into two groups randomly： the mice in the control group were injected intraperitoneally with normal saline at 3, 9, 15, and 21 HALO（Hours After Light Onset） for 3 days and the mice in the experimental group were injected intraperitoneally with CPT-11 66mg/ kg for 3 days at 3, 9, 15, and 21 HALO. The mice were sacrificed 15 days after the first injection. WBC, HB, and PLT levels in each mouse were assessed. Tumors were removed and the inhibitory rates were determined. Cell suspensions were prepared for detection of cell cycle changes by flow cytometry. The changes in intestinal mucosal tissue were observed with H＆E staining and light microscopy. Results： CPT-11 showed a significant therapeutic effect on nasopharyngeal carcinoma in the experimental group （P〈0.01）. The highest inhibitory rate of CPT-11 on nasopharyngeal carcinoma was in 15 HALO （P〈0.05） and the lowest was in 21 HALO （P〈0.05）. In 15 HALO, the WBC, HB, and PLT of mice in the experimental group were higher （P〈 0.05）, the body weight was increased more markedly and the grade of the intestinal mucosa damage was lower （P〈0.05） than those in the other experimental groups. In 21 HALO, the WBC, HB, and PLT of mice in the experimental group were the lowest （P〈0.05）, the body weight was decreased more markedly and the grade of the intestinal mucosa damage was higher （P〈0.05）. The carcinoma cells of 21 HALO and 3 HALO were mainly found to be in G2 phase （54.1100±17.33740,53.0300±20.7931）. Conversely, the carcinoma cells of 15 HALO and

关 键 词：OPT-11(艾力) 时辰化疗 人鼻咽低分化鳞癌细胞 设光后小时 

分 类 号：R739.63[医药卫生—肿瘤]