人疱疹病毒8型ORF75基因亚型与Kaposi肉瘤的相关性研究  

Stuay on the ORF75 subtypes of human herpesvirus-8 in patients with Kaposi＇ s sarcoma

作　　者：马春雷[1] 普雄明[1] 吴卫东[1] 张德志[1] 吴秀娟[1] 

机构地区：[1]新疆维吾尔自治区人民医院皮肤性病科,乌鲁木齐830001

出　　处：《中华皮肤科杂志》2009年第11期748-750,共3页Chinese Journal of Dermatology

摘　　要：目的探讨人类疱疹病毒8型（Httv-8）ORE75基因亚型，与Kaposi肉瘤不同临床分型及侵袭性的相关性。方法对25例新疆Kaposi肉瘤石蜡包埋组织进行HHV-8DNA抽提、扩增及双向测序，使用Clustal W软件和PHYLIP软件包对测序结果进行发生学分析，从而确定HHV-8ORF75基因亚型。结果25例Kaposi肉瘤中，21例HHV-8阳性，阳性率为84％，其中7例AIDS相关型Kaposi肉瘤患者HHV-8均阳性。21例HHV-8阳性患者中，18例为HHV-8 ORF75 A亚型，3例为C亚型；不同亚型间Kaposi肉瘤患者有无黏膜损害及临床分型的分布差异均无统计学意义（P〉0．05）。结论新疆Kaposi肉瘤患者感染HHV-8 ORF 75亚型属于A亚型和C亚型，HHV-8 ORF 75不同亚型可能与新疆Kaposi肉瘤黏膜损害及临床分型无关。Objective To profile the subtypes of open reading frame 75 （ORF75） of human herpesvims 8 （HHV-8） in patients with Kaposi＇s sarcoma, and to evaluate their relationship with clinical phenotypes and invasiveness of Kaposi＇ s sarcoma. Methods Twenty-five paraffin-embeded tissue specimens of Kaposi＇s sarcoma were collected in the Department of Dermatology, People＇s Hospital of Xinjiang Uygur Autonomous Region. DNA was extracted fi＇om these specimens, and nested-PCR was performed to amplify HHV-8 DNA followed by bi-directional sequencing. Phylogenetic analysis was carded out by using the software DNASTAR, Clustal W program and PHYLIP package so as to identify the ORF75 subtype of HHV-8. Results HHV-8 DNA was detected in 21 （84%） out of the 25 samples, and 7 cases of AIDS-associated Kaposi＇s sarcoma were all positive for HHV-8. Among the 21 patients carrying HHV-8 DNA, 18 were positive for subtype A ORF75, 3 for subtype C ORF75. The ORF75 subtypes had no significant correlation with the presence of rnucosal lesions or clinical phenotypes of Kaposi＇ s sarcoma. Conclusions The majority of patients with Kaposi＇s sarcoma in Xinjiang are infected with HHV-8 of ORF 75 subtype A and C. The ORF75 subtypes of HHV-8 have no correlation with the presence of mucosal lesions or clinical ohenotypes of Kanosi＇s sarcoma.

关 键 词：肉瘤 卡波西 疱疹病毒8型 人 开放读码框架 基因型 

分 类 号：R73[医药卫生—肿瘤]