干细胞标志物CD133、nestin和CD44在恶性黑素瘤皮损中的表达及意义  

Expressions of stem cell markers CD133, nestin and CD44 in malignant melanoma and their significance

作　　者：苏蓓蓓[1] 张江安[1] 于建斌[1] 高明[1] 徐文博[1] 

机构地区：[1]郑州大学第一附属医院皮肤科,450052

出　　处：《中华皮肤科杂志》2009年第11期751-753,共3页Chinese Journal of Dermatology

基　　金：河南省教育厅自然科学研究项目（2007320071）

摘　　要：目的探讨干细胞标志物CD133、nestin和CD44在恶性黑素瘤中的表达及意义。方法采用免疫组化SP法检测3种标记物在30例恶性黑素瘤及30例皮内痣皮损中的表达。结果CD133、nestin和CD44在恶性黑素瘤组织中的阳性表达率分别是53．33％，80．00％和20．00％，在皮内痣分别是23．33％，53．33％和0，两组各标志物阳性率差异比较均有统计学意义（P〈0．05）。CD133、nestin和CD44在恶性黑素瘤组织中阳性细胞率平均值分别为2．98％±5．62％，34．92％±34．89％和1．28％±3．26％，在皮内痣分别为0．10％±0．21％，7．26％±13．13％和0，两组各标志物差异比较均有统计学意义（P〈0．05）。两组中阳性细胞率与患者性别、年龄、病程比较，差异无统计学意义（P〉0．05）。结论干细胞标志物CD133、nestin和CD44在恶性黑素瘤中高表达，在皮内痣中低表达或不表达，提示恶性黑素瘤中可能存在肿瘤干细胞。Objective To investigate the expressions of stem cell markers CD133, nestin and CD44 in malignant melanoma and their significance. Methods Tissue samples were obtained from 30 patients with malignant melanoma and 30 patients with intradermal nevus. The expressions of three markers were immunohistochemically detected in the samples. Results In malignant melanoma specimens, the expression rate of CD133, nestin and CD44 was 53.33% （16/30）, 80.00% （24/30） and 20.00% （6/30）, respectively, significantly different from that in intradermal nevus specimens [23.33% （7/30）, 53.33% （16/30） and 0, respectively, all P 〈 0.05]. The percentage of cells positive for CD133, nestin and CD44 was 2.98% ± 5.62%, 34.92% ± 34.89% and 1.28% ± 3.26%, respectively, in malignant melanoma specimens, 0.10% ± 0.21%, 7.26% ± 13.13% and 0, respectively, in intradermal nevus specimens; there was a significant difference between the two groups of specimens （ all P 〈 0.05 ）. In malignant melanoma and intradermal nevus, the expression intensity of CD133, nestin and CD44 showed no significant correlation with patients＇ sex, age or disease course （all P 〉 0.05）. Conclusions CD133, nestin and CD44 are highly expressed in malignant melanoma, but weakly expressed or absent in intradermal nevus, suggesting that tumor stem cells might exist in malignant melanoma tissue.

关 键 词：黑色素瘤 实验性 干细胞 抗原CD133 抗原CD44 

分 类 号：R739.5[医药卫生—肿瘤]