Protection of Salvianolic Acid B for Human Endothelial Cells Against Hydrogen Peroxide-Induced Oxidative Damage  

作　　者：张俊刚 赵广荣 刘金玲 季祥武 

机构地区：[1]School of Chemical Engineering and Technology, Tianjin University [2]Department of Cardiology, Weifang People’s Hospital [3]Department of Endocrinology, Weifang Hospital of Traditional Chinese Medicine

出　　处：《Transactions of Tianjin University》2009年第6期434-439,共6页天津大学学报（英文版）

基　　金：Supported by National Natural Science Foundation of China (No30873400);Natural Science Foundation of Tianjin (No06YFJMC07300)

摘　　要：Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and concentration of glu-tathione were measured. Protective effect of Sal B on the endothelial cells from hydrogen peroxide-induced damage was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glu-tathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glu-tathione concentration after the oxidative challenge.Salvianolic acid B （Sal B） is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species （ROS）, activities of superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPX）, and concentration of glutathione were measured; Protective effect of Sal B on the endothelial cells from.hydrogen peroxide-induced damage was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glutathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glutathione concentration after the oxidative challenge.

关 键 词：salvianolic acid B redox status human endothelial cell hydrogen peroxide oxidative stress antioxidant enzyme reductive glutathione 

分 类 号：R363[医药卫生—病理学]