左旋精氨酸和超氧化物歧化酶联合对大鼠心肌缺血再灌注损伤的影响  

Influence of L-Arg combined SOD in the myocardial ischemia-reperfusion injury

作　　者：孟宪国[1] 周高峰[2] 

机构地区：[1]齐齐哈尔医学院附属第三医院内五科,161002 [2]吉林省长春市中医院心血管科

出　　处：《中国医药》2009年第12期965-967,共3页China Medicine

摘　　要：目的观察左旋精氨酸（L-Arg）及超氧化物歧化酶（SOD）在大鼠心肌缺血再灌注损伤中的作用。方法40只大鼠完全随机分为L—Arg组、SOD组、L—ArgSOD组及对照组各10只。在心肌缺血再灌注期间分别给予含L—Arg、SOD、L-Arg＋SOD及不含L—Arg和SOD的KH灌注液，期间测定心脏功能指标、乳酸脱氢酶、肌酸磷酸激酶、一氧化氮、心肌组织丙二醛含量。结果心肌缺血再灌注期间，各组间心率差异无统计学意义。L—Arg组左心室内压力高于对照组[分别为（71．2±3．7）mmHg和（52．3±4．3）mmHg]，冠状动脉流量也高于对照组[分别为（87．4±13．5）m]／min和（64．2±11．2）ml／min]（P〈0．05）；SOD组左心室内压力[分别为（74．3±3．4）mmHg]也高于对照组（P〈0．05），L—Arg＋SOD组左心室内压力[（78．6±3．2）mmHg]和冠状动脉流量高于L-Arg组和SOD组，差异有统计学意义（P〈0．05）。在缺血再灌注10min时，L—Arg组和SOD组乳酸脱氢酶、肌酸激酶均较对照组减少（P〈0．05），L—Arg＋SOD组乳酸脱氢酶、肌酸激酶均较L—Arg组和SOD组减少（P〈0．01）；L．Arg组冠状动脉流出液NO含量高于对照组（P〈0．05）；SOD组冠状动脉流出液NO含量和对照组相比差异无统计学意义；L-Arg＋SOD组略高于L-Arg组，但差异无统计学意义。与对照组相比，SOD组、L．Arg＋SOD组心肌丙二醛含量均明显降低（P〈0．01），但这2组间差异无统计学意义。L—Arg组心肌丙二醛含量和对照组相比，差异无统计学意义。结论L—Arg和SOD在心肌缺血再灌注损伤中有保护作用，二者联合效果更明显。Objective To observe the effect of L-arginine （L-Arg） and superoxide dismutase （SOD） on the myocardial ischemia-reperfusion injury. Methods KH perfusion fluid containing L-Arg, SOD and L-Arg ＋ SOD was given during the period of myocardial ischemi-reperfusion. Cardiac function,lactate dehydrogenase （LDH） , creatine phosphokinase （CPK）, Nitric oxide （NO） and the content of myocardial malondialdehyde （MDA） was observed. Results During the period of myocardial ischemia-reperfusion, left ventricular pressure （LVP） and coronary flow（CF） in L-Arg group was higher than those in control group. LVP in SOD group was higher than control group. At the same time, LVP and CF in L-Arg ＋ SOD group were significantly higher than those in L-Arg group and SOD group（P 〈0.05）. At 10 minutes after reperfusion, LDH and CPK in L-Arg group and SOD group were less than those in control group, while those in L-Arg ＋ SOD group were significantly less than L-Arg group and SOD group. Compared with control group, MDA level in SOD group and L-Arg ＋ SOD group were significantly lower（ P 〈 0.01 ）. Conclusion L-Arg and SOD alleviate the myocardial ischemia- reperfusion injury. A combined therapy showes better therapeautic effect than single use of L-Arg or SOD.

关 键 词：左旋精氨酸 超氧化物歧化酶 心肌缺血再灌注损伤 

分 类 号：R364.12[医药卫生—病理学]