不同激活剂条件下油藏内源微生物激活过程中DGGE分析  

Denature Gradient Gel Electrophoresis of Strata Bacteria Activation in Oilfield under Different Activating Nutrient Conditions

作　　者：包木太[1] 刘涛[1] 高光军[2] 郭辽原[2] 李希明[2] 

机构地区：[1]海洋化学理论与工程技术教育部重点实验室,中国海洋大学,山东青岛266100 [2]中国石化胜利油田分公司采油工艺研究院,山东东营257000

出　　处：《湖南大学学报（自然科学版）》2009年第11期67-72,共6页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(50604013)

摘　　要：在对胜利油田沾3区块油藏状况分析的基础上，模拟地层高温高压的条件利用分子生物学技术研究了不同激活剂对内源微生物的激活效果．实验分别以葡萄糖、蔗糖、玉米浆、淀粉作碳源，以油井产出水和注入水作为研究对象，进行微生物的激活优化．结果表明，激活以后水样中微生物种群发生了明显变化，以葡萄糖为碳源激活效果尤为突出．另改变葡萄糖的浓度对激活效果进一步研究，结果表明，当葡萄糖的浓度为5～7．5g·L^-1时较为合适，细菌密度能达到107cell．mL^-1．产出水的激活效果比注入水要好．On the basis of analyzing the reservoir status of ZHAN 3 block in Shengli Oilfield, different nutrients were used to activate the indigenous microorganisms by utilizing the denature gradient gel electrophoresis （DGGE） under the condition of stratum. The results showed that the microbial communities changed noticeably when glucose, cane sugar, corn steep liquor, starch solution were used as the carbon source ,and the produced water and injected water were used as the objects. In addition, based on the effect and the practicability, the concentration of glucose was altered in further experiments, which indicated that the density of the indigenous microorganisms could reach 107 cell · mL^-1 when the concentration of glucose was from 5 g · L^- 1 to 7.5 g · L^-1 , and the results also indicated that the produced water was better than the injected water for microbial growth.

关 键 词：内源微生物 油藏 脱氧核糖核酸 梯度凝胶变性电泳 激活剂 

分 类 号：X172[环境科学与工程—环境科学] TE337.9[石油与天然气工程—油气田开发工程]