高温合并化疗对耐药性大鼠肝癌CBRH-7919细胞多药耐药基因的影响  

Effect of hyperthermia and chemotherapy on CBRH-7919mdr MDR gene

作　　者：樊爱琳[1] 李怀平[2] 郑善銮[1] 简文[3] 杨麦贵[1] 王执民[4] 郝晓柯[1] 

机构地区：[1]第四军医大学西京医院检验科,陕西西安710038 [2]第四军医大学西京医院肾脏内科,陕西西安710038 [3]第四军医大学西京医院呼吸科,陕西西安710038 [4]第四军医大学唐都医院介入放射科,陕西西安710038

出　　处：《武警医学院学报》2009年第12期1005-1007,F0002,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】观察热化疗对多药耐药性CBRH-7919细胞MDR基因的影响。【方法】采用单纯化疗,单纯热疗及热疗联合化疗,分别作用于CBRH-7919及CBRH-7919mdr 1细胞。观察不同处理组细胞的存活率、mdr1mRNA及P-gp的表达情况。【结果】热化疗组细胞的存活率明显低于单纯加热组及单纯化疗组,同时热化疗组细胞mdr1mRNA的表达明显减低或消失,P-gp表达明显减低。【结论】热化疗可以使多药耐药细胞mdr1mRNA表达降低或消失,使P-gp表达降低,从而逆转了肿瘤细胞的多药耐药性,提高了化疗的敏感性。【Objective】To observe the effect of thermochemotherapy on CBRH-7919mdr cell MDR gene.【Methods】The effect of hyperthermia,chemotherapy and hyperthermia plus chemotherapy on CBRH-7919mdr1 cells were studied by observing the percentage of survival cells and the expression of mdr1mRNA and P-gp after thermochemotherapy.【Results】After hyperthermia puls ADM,a lower（or lost） expression of mdr1mRNA and lower expression of P-gp of CBRH-7919mdr 1 cells were found.Hyperthermia could intensify the effect of adriamycin to CBRH-7919mdr cells.【Conclusions】Thermochemotherapy can decline tumor cell′s expression of mdr1mRNA and P-gp,reverse tumor cell′s MDR and enhance the sensitivity of tumor cells to drugs.

关 键 词：鼠CBRH-7919细胞株 多药耐药性 P-糖蛋白 热化疗 

分 类 号：R735.7[医药卫生—肿瘤]