恶性骨肿瘤患者外周血单核细胞体外诱生NO和TNF-α的测定  

Determination and significance of nitric oxide and tumor necrosis factor α induced by peripheral blood monocyte in vitro in patients with malignant bone tumors

作　　者：肖涛 廖龙元 孙材江 王万春 李海声 方建珍 

出　　处：《湖南医科大学学报》1998年第6期596-598,共3页Bulletin of Hunan Medical University

摘　　要：分离１５例恶性骨肿瘤患者和１２例健康志愿者的外周血单核细胞，经脂多糖诱生培养４８ｈ，收集上清液；用Ｇｒｉｅｓｓ法及ＥＬＩＳＡ法测定其一氧化氮（ＮＯ）及肿瘤坏死因子－α（ＴＮＦ－α）的含量。结果发现：恶性骨肿瘤组ＮＯ和ＴＮＦ－α明显高于正常对照组，且二者呈高度正相关（ｒ＝０．８９０９，Ｐ＜０．０１）。Peripheral blood monocytes from 15 cases of malignant bone tumors and 12 cases of health volunteers were seperated and induced by lipopolysaccharide(LPS) for 48 hour culture. The supernatant was collected for determining the content of nitrite and tumor necrosis factor α(TNF α) (by Griess and ELISA methods, respectively). The results were as follows: The content of nitrite and TNF α in patients with malignant bone tumors was significantly higher than that in normal control; the content of nitrite and TNF α in the malignant bone tumor group presented the positive correlation (r=0.8909,P<0.01). The results suggest that NO and TNF α produced by activated macrophages may be related to the pathogenesis of malignant bone tumors.

关 键 词：骨肿瘤 一氧化氮 肿瘤坏死因子Α 单核细胞 

分 类 号：R738.1[医药卫生—肿瘤]