Fas/FasL系统在肿瘤细胞耐药中的作用  

The role of Fas/FasL ligand system in drug resistant cell line

作　　者：刘建夏[1,2] 张学光[1,2] 钱海鑫[1,2] 田力平 左剑玲[1,2] 

机构地区：[1]苏州医学院附属第一医院外科 [2]苏州医学院生物技术研究所

出　　处：《癌症》1998年第6期401-403,455,共4页Chinese Journal of Cancer

摘　　要：目的：探讨凋亡基因Ｆａｓ／ＦａｓＬ对肿瘤细胞耐药性的影响。方法：多发性骨髓瘤敏感株ＲＰＭＩ８２２６和耐药株ＲＰＭＩ８２２６／Ｄｏｘ４０分别和Ｄｏｘｏｒｕｂｉｃｉｎ、ＣＨ１１作用，在不同时间和浓度下观察二株细胞的Ｆａｓ／ＦａｓＬ的表达和死亡情况。结果：Ｄｏｘｏｒｕｂｉｃｉｎ能上调Ｆａｓ／ＦａｓＬ在敏感株肿瘤细胞表面的表达，从而激发ＰＣＤ，而耐药株则无此作用。结论：耐药细胞通过改变其Ｆａｓ／ＦａｓＬ系统的表达拮抗化疗药物引起的ＰＣＤ。Objective: Evaluating the effect of Apoptosis gene Fas/FasL in drugresistant cell lines. Methods: RPMI 8226 and RPMI 8226/Dox40 cells were treated with Dox and CH 11, Expression of Fas/FasL and Apoptosis were observed after treatment.Results: Dox induce Fas/FasL expression and apoptosis in RPMI 8226. But it shows least effects in RPMI 8226/Dox 40 cells. Conclusion: Fas/FasL system is involved with mechanism of drug resistance.

关 键 词：FAS/FASL 细胞凋亡 耐药性 骨髓肿瘤 

分 类 号：R733.305[医药卫生—肿瘤] R730.53[医药卫生—临床医学]