钙通道拮抗剂和钙调素拮抗剂对白血病耐药细胞的耐药基因和细胞内Ca^(2+)浓度的影响  

The effect of calcium cation and calmodulin antagonist on resistantgene of leukemia MDR cell and calcium cation concentration in cells

作　　者：陈同辛[1,2] 沈蕾[1,2] 林梓[1,2] 王耀平[1,2] 应大明[1,2] 

机构地区：[1]上海第二医科大学上海市儿科研究所 [2]上海第二医科大学附属新华医院

出　　处：《癌症》1998年第6期404-406,共3页Chinese Journal of Cancer

摘　　要：目的：探讨白血病耐药细胞的发生机制与细胞内钙离子浓度的关系。方法：分别用Ｆｕｒａ２／Ａｍ方法测定细胞内游离钙浓度及ｄｏｔ杂交法测定细胞ｍｄｒ１ｍＲＮＡ表达。结果：发现ＣＡｓ（Ｖｅｒ或Ｄｉｌ）有降低肿瘤细胞内Ｃａ２＋浓度和降低ｍｄｒ１ｍＲＮＡ表达的作用，ＣａＭＡｓ虽无降低Ｃａ２＋浓度的作用，但可使ｍｄｒ１ｍＲＮＡ表达降低，从而降低了Ｋ５６２／ＶＣＲ细胞的耐药性。结论：细胞内Ｃａ２＋浓度的变化可能与ＭＤＲ产生有一定关系；ＣＡｓ导致的Ｃａ２＋浓度的变化可能也是其逆转肿瘤细胞ＭＤＲ的机理之一。Purpose: To study the mechanism of the Multi drug resistant cells and the relationship to the calcium cation level in cells. Methods: the effect of CAs and CaMAs on intracellular Ca2+ concentation was determined and mdrl mRNA expression level of tumor cells with spectrofluorometry and Dot hybridization technique was used. Results: the results showed the calcium antagonists (Ver. and Dil) have the potential to decrease the calcium cation level within the tumor cells and the Mdrl mRNA expression. Although the CaMAs hasn't the potential to decrease the calcium cation level within cells, still has the potential to decrease the Mdrl mRNA expression thus lower the K562/VCR cell line's multi drug resistance. Conclusions: The change of intracellular Ca2+ concentration could be related to the production of MDR, the effect of CAs on intracellular Ca2+ may be one of the mechanisms of MDR reversal in cancer cells; CAs and CaMAs could also affect the mdrl mRNA expression.

关 键 词：白血病 耐药基因 钙调素拮抗剂 钙通道拮抗剂 

分 类 号：R733.7[医药卫生—肿瘤] R972[医药卫生—临床医学]