反义Gαq/11亚基ODNs对平滑肌细胞DNA合成和PC蛋白表达的影响  

Effects of antisense Gαq/11 subunits oligodeoxynucleotides on the DNA synthesis and expression of proliferating cell nuclear antigen of rat vascular smooth muscle cell

作　　者：李朝红[1] 邓漪平[1] 刘青[1] 刘素红[1] 

机构地区：[1]中山医科大学组胚心血管病分子生物学组

出　　处：《中国病理生理杂志》1998年第6期599-602,共4页Chinese Journal of Pathophysiology

摘　　要：目的：探讨Ｇｑ蛋白介导血管活性多肽对ｖＳＭＣＤＮＡ合成及增殖细胞核抗原（ＰＣＮＡ）蛋白表达的影响。方法：用硫代修饰的Ｇαｑ／１１亚基反义寡聚脱氧核苷酸（Ｇαｑ／１１ＡＳ－ＯＤＮｓ），以６μｍｏｌ／Ｌ的浓度加入含１０－７ｍｏｌ·Ｌ－１ＥＴ－１刺激无血清ＤＭＥＭ培养基中体外培养ｖＳＭＣ。用３Ｈ－ＴｄＲ掺入法测定ｖＳＭＣＤＮＡ合成、免疫细胞化学技术检测ｖＳＭＣＰＣＮＡ蛋白表达。结果：Ｇαｑ／１１ＡＳ－ＯＤＮｓ可明显抑制ｖＳＭＣＤＮＡ的合成，在１２ｈ，２４ｈ时抑制率分别为８４２％和８５３％；Ｇαｑ／１１ＡＳ－ＯＤＮｓ亦明显降低ｖＳＭＣＰＣＮＡ蛋白的表达。而相同浓度的正义Ｇαｑ／１１ＯＤＮｓ只呈现少量抑制作用。Abstract AIM:In order to explore the effect of Gαq/11 subunits mediating numerous vascular bioactive polypeptides on DNA synthesis and expression of proliferating cell nuclear antigen(PCNA)in vSMC.METHODS:Antisense Gαq/11 subunits oligodeoxynucleotides (Gαq/11AS-ODNs)were used at a concentration of 6 μmol/L in the serum-free DME culture medium containing ET-1(10 -7 mol·L -1 )for vSMCs culture.By means of 3H - thymidine incorporation and immunocytochemistry,DNA synthesis and expression of PCNA in vSMC were detected.RESULTS:Gαq/11 AS-ODNs significantly blocked the incorporation of 3H-thymidine in vSMC.Inhibitory rates were 84.2% and 85.3% at 12h,24h culture respectively.Gαq/11 AS-ODNs also markedly reduced the expression of PCNA in vSMCs.While Gαq/11 sense ODNs only had a little effect as a control.CONCLUSION:The results indicate that Gαq/11 AS-ODNs may be useful for the study of prevention and treatment of cell proliferative diseases induced by vasoactive polypeptides. MeSH G-proteins;Oligonucleotides,antisense;Muscle,smooth,vascular;Endothelins

关 键 词：G蛋白 VSMC 基因表达速度 血管平滑肌增殖 

分 类 号：R543.02[医药卫生—心血管疾病]