人胚脑皮层N-甲基-D-门冬氨酸受体亚单位蛋白的表达  

Developmental expressions of N-methy1-D-aspartate (NMDA) receptor NR1, NR2A, and NR2B subunit proteins in cortex of human embryos

作　　者：罗建红[1,2] 朱丽君[1,2] 陈杰 

机构地区：[1]浙江医科大学医学分子生物学实验室 [2]浙江省金华市中心医院

出　　处：《中国病理生理杂志》1998年第6期612-616,共5页Chinese Journal of Pathophysiology

摘　　要：目的和方法：采用ＮＭＤＡ（Ｎ－甲基－Ｄ－门冬氨酸，Ｎ－ｍｅｔｈｙｌ－Ｄ－ａｓｐａｒｔａｔｅ）受体ＮＲ１、ＮＲ２Ａ及ＮＲ２Ｂ三种亚单位特异的抗体，对不同胎龄（１２～３３周）的人胚及成人脑皮层组织的这三种亚单位蛋白作了免疫印迹分析。结果：发现ＮＲ１、ＮＲ２Ａ及ＮＲ２Ｂ三种亚单位蛋白在成人脑皮层组织均有表达；１２周龄的人胚脑皮层组织就有ＮＲ１和ＮＲ２Ｂ亚单位蛋白的表达，随着胎龄增大此二种蛋白表达量随之增高，至３３周龄时二者的含量达成人水平的７５％左右；然而在上述所有人胚脑皮层样本中未能检出ＮＲ２Ａ亚单位蛋白。结论：人胚脑皮层组织具有ＮＲ１和ＮＲ２Ｂ亚单位蛋白的表达。Abstract AIM and METHOD:By using quantitative immunobloting with NMDA receptor NR1, NR2A and NR2B subunit-specific antibodies, the protein levels of the three subunits have been determined in cortex tissues of human embryos aged between 12w～33w, as well as adult human cortex.RESULTS:NR1 and NR2B subunit proteins express in cortex of human embryos at least as early as 12 weeks of pregnancy, and the expressions of both the subunits have a same pattern of increase as embryos grow up. On the other hand, NR2A subunit protein is undetectable in all of the human cortex samples from the embryos aged between 12 to 33 weeks. CONCLUSION: Cortex of human embryos expresses NR1 and NR2B subunit proteins of NMDA receptor with increasing pattern at the developmental stage. MeSH Human;Embryo; Cerebral cortex; Immunobloting

关 键 词：胚胎 大脑皮层 免疫印迹法 NMDA 受体 突触 

分 类 号：R338.13[医药卫生—人体生理学] R321.5[医药卫生—基础医学]