氢氧化钠预处理和酶解牛粪纤维素工艺条件优化  

Optimization of Technical Conditions for NaOH Pretreatment and Enzyme Hydrolysis of Cellulose in Cow Dung

作　　者：徐静静[1] 李静[1,2] 凌娟[1] 刘茂昌[1] 燕飞来[1] 

机构地区：[1]西南大学资源环境学院,重庆400716 [2]重庆市农业资源与环境研究重点实验室,重庆400716

出　　处：《山东农业科学》2009年第11期77-79,共3页Shandong Agricultural Sciences

基　　金：重庆市北碚区科委科技计划项目(2007-13);重庆市科委自然科学基金计划资助项目(CSTC;2006BB7335);西南大学博士基金项目资助

摘　　要：对氢氧化钠预处理牛粪中纤维素的工艺条件进行了优化研究,各因素较优的水平组合为处理温度95℃、时间3 h、碱浓度3%、固液比1∶12.5。氢氧化钠预处理后,纤维素在总体中所占比例有较大提高,而半纤维素和木质素则有所降低。之后经纤维素酶处理,各因素较优的水平组合为温度55℃、酶液浓度10g/L、底物浓度25 g/L、pH值4.4。The conditions for degradation of cellulose in cow dung by NaOH pretreatment and enzyme hydrolysis were optimized.The optimum conditions for NaOH pretreatment were the temperature of 100℃,3-hour treatment,the NaOH concentration of 3% and the solid-liquid ratio of 1︰12.5.After the NaOH pretreatment,the cellulose proportion increased greatly,while the proportion of hemicellulose and lignin reduced.Then the optimum conditions for enzyme hydrolysis were the temperature of 55℃,the enzyme concentration of 10 g/L,the substrate concentration of 25 g/L and pH 4.4.

关 键 词：牛粪 纤维素 碱预处理 酶解 

分 类 号：X713[环境科学与工程—环境工程]