榄香烯对胶质瘤细胞U251的作用机制  

Mechanism about Elemene antitumor activity on glioma cell

作　　者：程秀菊[1] 魏守刚[1] 刘海燕[2] 张永法[1] 许致胜[1] 

机构地区：[1]潍坊市益都中心医院儿科,山东青州262500 [2]山东大学齐鲁医院儿科

出　　处：《肿瘤研究与临床》2009年第11期721-723,727,共4页Cancer Research and Clinic

摘　　要：目的从细胞水平和分子水平探讨榄香烯对胶质瘤细胞的作用机制。方法取胶质细胞瘤的U251细胞株做体外培养，四甲基偶氮唑盐比色（MTT）法检测榄香烯对人神经胶质瘤细胞U251的增生抑制作用，流式细胞术（FCM）检测细胞周期、细胞内钙离子（IECa^2＋）含量、Fas、bcl-2、增生细胞核抗原（PCNA）蛋白表达及细胞凋亡。结果榄香烯能有效抑制胶质瘤细胞U251的恶性增生，MTT法检测榄香烯处理不同时间（24、48、72h）的半数生长抑制剂量（IC50），24、48、72h其IC50分别为40．60、38．14、34．35μg／ml。经榄香烯作用后，细胞周期可阻滞在S期和G2／M期。用异硫氰酸胍荧光素（Annexin V FITC）染色检测发现细胞凋亡率明显增加。榄香烯还可下调PCNA、Fas蛋白的表达，上调细胞内Ca^2＋（IECa^2＋）含量，对bcl-2蛋白表达无显著影响。结论榄香烯有显著的抗胶质瘤细胞增生作用，并能通过下调PCNA蛋白的表达，上调细胞内Ca^2＋含量，诱导细胞凋亡，对Fas表达也有影响，可能与bcl-2基因表达无关。Objective To explore the activity of Elemene for glioma cell from the cellular and molecular level. Methods The human glioma cell U251 was cultured. The effect of Elemene for human glioma cell proliferation was studied by MTT assay. Cell cycle, Fas, PCNA, bcl-2, intracellular Ca^2＋ and apoptosis were evaluated by flow cytometry analysis. Results Elemene exhibited antiproliferative effect on human glioma cell U251 markedly. The fifty percent inhibition on concentration （IC50） of Elemene against glioma cells at different time points. 24 h was 40.60 μg/ml, the 48 h 38.14 μg/ml and the 72 h 34.35μg/ml. Cell cycle was blocked in the S and G2/M phases. The apoptosis ratio was increased by Annexin V staining markedly. Elemene decreased the gene expressions of PCNA and Fas, increased the intracellular Ca^2＋. There was no significant effect on the bcl-2 gene expression. Conclusion Elemene exhibits a marked antiproliferative effect on glioma cells and induces apoptosis by decreasing the expression of PCNA and increasing intracellular Ca^2＋. It also influences the expression of Fas. It might have no relationship with bcl-2 gene expression.

关 键 词：榄香烯 神经胶质瘤 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]