肺癌患者自然杀伤细胞活性受体NKG2D与可溶性MICA的表达及其相关性分析  

Expression and correlation of NKG2D and sMICA in lung cancer patients

作　　者：陈钢[1] 王春利[1] 郭石平[1] 苏文[2] 乔丽娟[1] 麦羡霞[1] 马捷[3] 胡成广[1] 张沛刚 

机构地区：[1]山西省肿瘤医院胸外二科,太原030013 [2]山西省肿瘤研究所免疫室 [3]山西医科大学第二医院心胸外科 [4]山西省汾阳医院胸外科

出　　处：《肿瘤研究与临床》2009年第11期759-761,共3页Cancer Research and Clinic

摘　　要：目的探讨肺癌患者自然杀伤细胞活性受体（NKG2D）与可溶性MICA（sMICA）的表达及其二者的相关性。方法选取30例肺癌患者作为试验组，30例健康志愿者作为对照组，采用流式细胞术（FCM）与酶联免疫吸附（ELISA）法分别测定两组NKG2D与sMICA分子的表达。结果肺癌患者和健康人群NKG2D分子表达分别为（81．56±8．78）％、（85．63±6．62）％，二者差异有统计学意义（P〈0．05）。肺癌患者和健康对照血清中sMICA分子表达分别为（354．13±80．575）pg／ml、（216．53±48．175）pg／ml，二者差异有统计学意义（P〈0．01）。NKG2D和血清中sMICA分子两个变量之间具有明显相关性（r=-0．349，P=0．006），呈负相关关系。结论NKG2D的表达和血清中sMICA的水平可能成为预测肺癌患者免疫功能状态及肿瘤恶性转移的指标之一。sMICA表达及对NKG2D的负调节机制可能是导致肿瘤细胞发生免疫逃逸的原因之一。该机制可能会为肿瘤生物免疫治疗提供患者适应证指标。Objective To investigate the expression and correlation of NKG2D and sMICA in lung cancer patients. Methods By collecting 30 lung cancer patients as the test group,and taking 30 heah.hy volunteers as the contrast group, the expression of NKG2D and sMICA in the two groups were examined separately by FACS and ELISA method. Results The expressions of NKG2D in the two groups were （81.56± 8.78） %, （85.63±6.62） %. The lung cancer patients were high remarkable. There was a significant difference between the two groups（P 〈0.05）. The expression of sMICA in the two groups were （354.13±80.575） pg/ml, （216.53±48.175） pg/ml. The lung cancer patients were low remarkable. There was a significant difference between the two groups （P 〈0.01）. There was a significant relation between the two groups （r =-0.349, P = 0.006）. Conclusion The expression of NKG2D and sMICA may provid one of the immune targets for diagnosing that can forecast the immune state and malignant metastasis of the lung cancer patients. The significant relation between NKG2D and sMICA may take on main role in the immune escaping of tumor. It may provide the suitable target of the patients for tumor organisms and immune treatment.

关 键 词：肺肿瘤 受体 神经激肽2 基因 MHC Ⅰ类 免疫逃逸 肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]