免疫组化鸡尾酒法检测P504S、P63和34βE12在前列腺病变诊断中的意义  

Significance of Using a P504S,P63,34βE12 Cocktail for the Diagnosis of Prostate Lesion

作　　者：李超霞[1] 潘文海[1] 杨小红[1] 方力[1] 陈辉[1] 华兴[1] 

机构地区：[1]暨南大学医学院第四附属医院广州市创伤外科研究所,广东广州510220

出　　处：《癌变．畸变．突变》2009年第6期431-434,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省社会发展领域科技计划项目(63101)

摘　　要：背景与目的:探讨在前列腺穿刺活检标本组织中联合检测α-甲酰基辅酶A消旋酶(P504S)、P63蛋白和高分子量角蛋白(34βE12)对前列腺良恶性病变诊断及鉴别诊断的意义。材料与方法:采用组合式单克隆抗体和双酶标记的免疫组化鸡尾酒法检测32例前列腺癌(PCa)、16例前列腺不典型腺瘤样增生(AAH)和44例良性前列腺增生(BPH)穿刺活检标本,在同一张组织切片上检测P504S、P63和34βE12抗原的表达情况。结果:PCa中P504S均呈阳性表达,阳性率100%,而P63和34βE12呈阴性表达;AAH中上述3种蛋白的阳性表达率均为75%;BPH中P504S均呈阴性表达,而P63、34βE12均呈阳性表达,阳性率100%;P504S、P63和34βE12抗原在PCa、AAH、BPH 3组标本中的表达两两比较差异均有统计学意义(P<0.05)。结论:免疫组化鸡尾酒法可作为前列腺疾病诊断及鉴别诊断的一种有效辅助手段;P504S是PCa高度敏感和特异的标志物,P63和34βE12联合标记基底细胞的特异性高,3者联合检测能提高前列腺穿刺活检标本诊断的准确性。BACKGROUND AND AIM： To examine the diagnosis of prostate lesion in needle biopsy specimen. significance of P504S, p63, 34βE12 cocktail in the differential MATERIALS AND METHODS： The expressions of P504S, P63 and 34βE12 were examined in the biopsy specimens of 32 prostate eancer（PCa）, 16 prostatic atypical adenomatous hyperplasia （AAH）and 44 benign prostatic hyperplasia （BPH） using immunohistoehemieal method with triple-antibody cocktail （P504S, P63, 34βE12） staining and double-color ehromogens in single paraffin sections. RESULTS： PCa stained positive for P504S and negative for P63 and 34βE12, and the positivity rate of P504S was 100%. The positive rates in AAH were all 75% for the three proteins. BPH strongly expressed P63 and 34βE12, but not P540S. The expressions of P504S, P63 and 34βE12 were significantly different among PCa, AAH and BPH （P 〈 0.05） . CONCLUSION： Cocktail staining may be an effective approach in the diagnosis and differential diagnosis of prostate lesion .P504S was a sensitive and specific marker for PCa. P63 and 34βE12 cocktail staining could increase the sensitivity and specificity for the basal cell layer. The triple-antibody cocktail staining could improve diagnostic accuracy of prostate lesion in needle biopsy specimen, and important for the early diagnosis of prostate cancer.

关 键 词：前列腺癌 Α-甲酰基辅酶A消旋酶 P63 34ΒE12 免疫组化 

分 类 号：R737.25[医药卫生—肿瘤]