Humanin拮抗Aβ_(31-35)诱导的神经元凋亡  

Humanin protects neurons against apoptosis induced by Aβ_(31-35)

作　　者：李灵敏[1] 刘青华[1] 张宇[2] 乔健天[2] 张策[2] 

机构地区：[1]山西医科大学病理学教研室,太原030001 [2]山西医科大学生理学教研室,太原030001

出　　处：《神经解剖学杂志》2009年第6期626-630,共5页Chinese Journal of Neuroanatomy

基　　金：山西省自然科学基金(2007011111)资助项目

摘　　要：为了研究Humanin(HN)对Aβ31-35诱导的大鼠皮层神经元凋亡的影响,本研究采用原代培养的大鼠皮层神经元,应用流式细胞术、TUNEL法、HO33342染色法检测不同时间(0、8、16h)加入不同浓度的HN对Aβ31-35致神经元凋亡的影响。结果显示:Aβ31-35(25μmol/L)引起培养皮层神经元的凋亡率明显增高,神经元凋亡率由7.43%上升到32.69%;凋亡指数由6.87%上升到28.36%(P<0.05)。与Aβ31-35同时或提前8h给予不同浓度(5,10,20μmol/L)的HN对Aβ31-35(25μmol/L)诱导的神经元凋亡均未产生影响;但20μmol/L的HN提前16h孵育可明显抑制Aβ31-35所致的神经元凋亡,其凋亡率由32.69%下降到20.36%,凋亡指数由28.36%下降到17.57%。本研究结果提示,HN拮抗Aβ31-35致神经元凋亡作用具有剂量和时间依赖性。To investigate the effects of Humanin （HN） on neuronal apoptosis induced by Aβ31-35, cultured cortical neurons were pretieated with different concentration of HN for various time period （0,8, 16 h） respectively, and then exposed to Aβ31-35 for additional 24 h and the neuronal apoptosis were examined by flow-cytometric assays, TUNEL staining and H033342 staining. The results showed that when neurons were pretreated with AI331-35 （25μmol/L）, the apoptotic rate was enhanced from 7.43% to 32.6%, and apoptotic index （AI） increased from 6.87% to 28.36% , respectively（P 〈 0.05 ）. HN produced no significant changes, when different concetration of HN （5 μmol/L, 10 μmol/L, 20μmol/L） were introduced simitaneously with A[331-35 or 8 h before exposure to Aβ31-35,respectively. However, higher concentration of HN （20μmol/L） administered 16 h in advance significantly suppressed Aβ31-35 nduced apoptosis from 32.69% to 20.36% ,and from 28.36% to 17.57% for AI. These results suggest that HN protected cultured cortical neurons against apoptosis induced by Aβ31-35 and the protective effect of HN is time-dependent and dose-dependent.

关 键 词：HUMANIN Aβ31-35 凋亡流式细胞术TUNEL大鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]