D-半乳糖调控SD大鼠大脑皮质巯醇抗氧化物(酶)致衰老的作用研究  

D-galactose regulates thiol antioxidants of cerebral cortex and induces aging in SD rats

作　　者：李守民[1] 王婷婷[2] 艾青龙[2] 李兴国[1] 傅希玥[1] 郭家智[1] 陆地[1] 孙俊[1] 

机构地区：[1]昆明医学院人体解剖学教研室,昆明650031 [2]昆明医学院昆明医学院附一院神经内科,昆明650032

出　　处：《神经解剖学杂志》2009年第6期651-654,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然基金项目(30860336);云南省科技厅-昆明医学院联合项目(2008C0054R,2007C0005R);云南省教育厅科学研究基金项目(05Z0355C)资助项目

摘　　要：构建D-半乳糖(D-gal)致衰老模型,探讨D-gal诱导衰老的分子机制,为临床治疗阿尔茨海默病(AD)提供理论依据。腹腔注射D-gal构建SD大鼠衰老模型,采用Morris水迷宫实验(MWM)进行行为学检测,采用化学比色法检测大脑皮质一氧化氮合酶(NOS)、一氧化氮(NO)、铜锌-过氧化物岐化酶(Cu,Zn-SOD)、总超氧化物歧化酶(T-SOD)、谷胱甘肽还原酶(GR)、谷胱甘肽s-转移酶(GSH-ST)、谷胱甘肽过氧化酶(GSH-PX)、谷胱甘肽(GSH)、丙二醛(MDA),总抗氧化能力(T-AOC)和过氧化氢(H2O2)的含量。结果显示:衰老模型组与正常对照组比较:逃避潜伏期明显延长(P<0.05),在第III象限逗留的时间明显减少(P<0.05),跨越平台次数明显减少(P<0.05);皮质MDA、H2O2和NO的含量增加(P<0.05),SOD、GSH、GSH-ST、GSH-Px和T-AOC的含量降低(P<0.05),而NOS的含量没有明显变化(P>0.05)。结果提示,D-gal能通过调控内源性巯基抗氧化物(酶)和NO的表达,减退学习记忆能力,而诱导SD大鼠神经系统的衰老。To investigate the molecular mechanisms of the aging induced by D-galactose, we used D-galactose- aging rat model, so as to provide basic theory for treating Alzheimer＇s disease （AD） in clinic. Aging rat model was established by injecting peritoneally D-Galactose （ 100 mg/kg, 56 days） into the rats. The behaviour was examined by the Morris water maze （MWM）. The nitric oxide synthase （NOS） , nitric oxide （ NO）, Cu Zu-superoxide dismutase （ Cu Zn-SOD） , total superoxide dismutase （T-SOD） , glutathione reductase （GR） , Glu- tathione-s-transferase （ GSH-ST）, glutathione peroxidase（ GSH-Px）, glutathione （ GSH）, malondialdehyde （ MDA）, anti-oxidative capabilities （T-AOC） and hydrogen peroxide （H2 02 ） activities were examined by colorimetric method. The results showed that the model rats exhibited significant increase in escape latencies （ P 〈 0.05 ）, while a decrease in the time of staying in quadrant of platform and the num- ber of crossing over a platform as compared to control group （ P 〈0.05 ）. The cortex of the model rats showed significant increase in MDA, H2O2 and NO activities（P 〈 0.05）, while a decrease in T-AOC, T-SOD, GSH, GSH-Px and GSH-ST activities as compared to control group （ P 〈 0.05 ）. However, the difference of NOS activity was not detected between two groups （ P 〉 0.05 ）. These present results suggest that Chronic D-galactose exposure can injure the spatial memory and induce the aging of central nervous system by manipulating thiol an- tioxidants and NO of the cerebral cortex in SD rats.

关 键 词：D-半乳糖 巯醇抗氧化物(酶) 一氧化氮 衰老 

分 类 号：R749.16[医药卫生—神经病学与精神病学]