二苯乙烯苷通过抑制氧化应激对MPP^+诱导的PC12细胞凋亡的保护作用  

TSG protects PC12 cells against MPP^+-induced apoptosis via the inhibition of oxidative stress

作　　者：李晓冰[1] 陈建宗[1] 李晓峰[1] 孙欣[1] 

机构地区：[1]第四军医大学西京医院中医药研究中心,西安710032

出　　处：《神经解剖学杂志》2009年第6期667-671,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30772743)资助项目

摘　　要：为了探讨2,3,5,4’-四羟基二苯乙烯-2-ο-β-D-葡萄糖苷(2,3,5,4’-tetrahydroxystibene-2-ο-β-D-glucoside,TSG)对1-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinium,MPP+)诱导PC12细胞凋亡的影响及其可能机制,本实验分为对照组,MPP+处理组和TSG(1、5和10μmol/L)预处理组。用Hoechst33258染色法和流式细胞术测定细胞凋亡,对活性氧(reactive oxygen species,ROS)敏感的荧光探针2,7-dichlorofluorescin dictate(DCF-DA),丙二醛(malondialdehyde,MDA)和总抗氧化能力(total anti-oxida-tion competence,T-AOC)检测试剂盒测定细胞的氧化应激的变化。结果显示:TSG三个浓度预处理后,对比MPP+处理组,观察到TSG在一定范围内以剂量依赖方式,使细胞核凝聚明显减少,细胞凋亡率降低。另外,TSG预处理后,PC12细胞中增高的ROS和MDA水平较MPP+处理组明显有所减低,T-AOC有所增强。以上结果提示,TSG可抑制MPP+诱导的PC12细胞的凋亡,其机制可能与TSG抑制氧化应激有关。To observe the effect of 2,3,5,4＇-tetrahydroxystibene -2-o-β-D-glucoside （TSG） on the PC12 cells apoptosis induced by 1- methyl-4-phenylpyridinium （MPP ＋ ） and its possible mechanism,the PC12 cells were divided into control group, MPP ＋ group and TSG pre-treatment group with different concentrations （ 1,5 and 10μmol/L） in this study. The Hoechst33258 staining and flow cytometry were employed to observe the apoptosis in PC12 cells. 2, 7-dichlorofluorescin dictate （DCF-DA） , an reactive oxygen species （ROS）-sensitive fluorescent probe, malondialdehyde （MDA） and total anti-oxidation competence （ T-AOC ） assay kit were used to detect the changes of oxidative stress. The results showed that TSG reduced the ratio of condensed nuclei, decreased the apoptosis percentage after TSG pre-treat- ment with three different concentrations, compared with the MPP ＋ -treated cells. In addition, the increase of the ROS and MDA level was reduced and the T-AOC was stronger by the pre-treatment with different concentrations of TSG, compared with the MPP ＋ -treated cells. The above results suggest that TSG could inhibit the apoptosis induced by MPP ＋ in PC12 cells, the possible mechanisms might correlate with the antioxidant activity of TSG.

关 键 词：二苯乙烯苷 MPP+ PC12细胞 凋亡 氧化应激 

分 类 号：R742.5[医药卫生—神经病学与精神病学]