磁共振导引靶向注射rAd-p53治疗兔VX2脑瘤实验研究  

The experimental study of rAd-p53 in treatment of rabbits VX2 brain tumors guided by MRI

作　　者：谢国华[1,2] 刘永波 李成利[1] 武乐斌[1] 张传臣[2] 柳明[1] 吕玉波[1] 李雷[1] 包守刚[1] 

机构地区：[1]山东省医学影像学研究所磁共振介入室,山东济南250021 [2]山东省聊城市人民医院,山东聊城252000 [3]山西省潞安矿业集团总医院,山西潞安047500

出　　处：《医学影像学杂志》2009年第11期1473-1476,共4页Journal of Medical Imaging

摘　　要：目的:探讨磁共振导引下注射rAd-p53治疗兔脑VX2瘤的可行性研究。方法:12只新西兰大白兔随机分为对照组(A组)、治疗组(B组)两组,A、B组各为6只,全麻下通过颅骨钻孔种植瘤块的方法建立VX2移植性脑瘤模型。脑瘤生长至直径平均约0.5cm时,在磁共振导引下穿刺肿瘤并分别靶向注射0.5ml无菌生理盐水(A组)和0.5ml rAd-p53(B组),随后各组分别在术后1天、3天、5天行MRI扫描后每组处死2只,行病理学及免疫荧光检查。结果:12只兔全部有脑瘤生长,且肿瘤大小较一致,形态规则,MR扫描呈T1WI略低信号,T2WI呈略高信号;增强扫描(钆喷替酸葡甲胺0.1mmol/kg体重)呈明显较均匀强化,肿瘤最大直径0.5~0.7cm,和病理学表现相一致,能够满足介入治疗的需要。所有注射无菌生理盐水和rAd-p53的介入操作均成功进行,术后1、3、5天病理学及MRI均未观察到明显差异,但在免疫荧光上,B组P53蛋白表达及凋亡均明显强于A组。结论:MRI导引下脑瘤注射rAd-p53治疗方法可行,能提高P53蛋白表达率及肿瘤凋亡率。Objective： To evaluate the feasibility of MRI-guided injection rAd-p53 to the VX2 brain tumor of rabbit. Methods： 12 New Zealand white rabbits were randomly divided into control group （group A）, treatment group （group B）, with 6 rabbits in each group. Un- der general anesthesia through the skull hole of planting methods, VX2 transplanted brain tumor model was established. The rabbits in group A and group B were implanted VX2 brain tumor respectively. When tumors about 0.5 cm in diameter, the MRI-guided injection of 0.5 ml sterile saline and 0.5 ml rAd-p53 were performed respectively. Subsequently, group A, B after 1, 3, 5 days were examined by then two rabbits in each group were killed and pathological examination and immunofluorescenee were performed. Results： Brain tumor grew in aec 12 rabbits. The tumors showed slight low signal on T^WI and slight high signal on T2WI as well as contrast enhance- ment after injection of Gd-DTPA. The maximum diameter of brain tumor was from 0.5 to 0.7 cm. Over the same period. MRI showed a consistent tumor size and shape. Mill findings were in line with pathological elmnges. It could meet the needs of intervenfional therapy. The procedures of sterile saline and rAd-p53 injection were all performed successhtlly. Pathology and MILI manifestation had no significant differences after postoperative 1,3,5 days. But in immtmofluorescence, F53 protein expression and apoptesis in group B were significantly saperior to group A. Conclusion： It is safe and feasibility of rAd-p53 injection into brain tumor under MR_I-guidance, which could signifi- cantly increase the P＇53 protein expression and tumor apoptosis.

关 键 词：磁共振介入 兔 动物模型 脑瘤 RAD-P53 VX2瘤 免疫荧光 

分 类 号：R445.2[医药卫生—影像医学与核医学] R739.41[医药卫生—诊断学]