Genetic mapping of quantitative trait loci associated with β-amylase and limit dextrinase activities and β-glucan and protein fraction contents in barley  

作　　者：Kang WEI Da-wei XUE You-zong HUANG Xiao-li JIN Fei-bo WU Guo-ping ZHANG 

机构地区：[1]Department of Agronomy, Zhejiang University, Hangzhou 310029, China [2]College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China [3]College of Agriculture, Guangxi University, Nanning 530004, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2009年第11期839-846,共8页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (Nos. 30630047 and 30771281);the Hi-Tech Research and Development Program (863) of China (No. 2006AA10Z1C3);the Ministry of Education and the State Administration of Foreign Experts Affairs (111 Project) of China (No. B06014)

摘　　要：High malting quality of barley （Hordeum vulgare L.） relies on many traits, such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study, interval mapping was utilized to detect quantitative trait loci （QTLs） affecting these malting quality parameters using a doubled haploid （DH） population from a cross of CM72 （six-rowed） by Gairdner （two-rowed） barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H, 4H, 5H, and 7H. Five of the nine QTLs mapped to chromosome 3H, indicating a possible role ofloci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for D-glucan and limit dex- trinase were identified on chromosomes 4H and 5H, respectively. QTL for hordeins was coincident with the region of silica eluate （SE） protein on 3HS, while QTLs for albumins, globulins, and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to （J-amylase, and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.High malting quality of barley (Hordeum vulgare L.) relies on many traits,such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study,interval mapping was utilized to detect quantitative trait loci (QTLs) affecting these malting quality parameters using a doubled haploid (DH) population from a cross of CM72 (six-rowed) by Gairdner (two-rowed) barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H,4H,5H,and 7H. Five of the nine QTLs mapped to chromosome 3H,indicating a possible role of loci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for β-glucan and limit dextrinase were identified on chromosomes 4H and 5H,respectively. QTL for hordeins was coincident with the region of silica eluate (SE) protein on 3HS,while QTLs for albumins,globulins,and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to β-amylase,and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.

关 键 词：Barley （Hordeum vulgate L.） Quantitative trait locus O-amylase Limit dextrinase Β-GLUCAN Protein fraction 

分 类 号：S511[农业科学—作物学] TS262.5[轻工技术与工程—发酵工程]