Canstatin蛋白在结肠癌中的表达及其重组蛋白抗结肠癌作用  

Canstatin gene expression in human colorectal carcinoma tissues and the anti-tumor effects of the recombinant canstatin protein

作　　者：王力[1] 马艳[2] 焦东晓[3] 王淑芬[2] 

机构地区：[1]南华大学附属第一医院普外科,湖南衡阳421001 [2]南华大学附属第一医院妇产科,湖南衡阳421001 [3]南华大学附属第一医院肿瘤外科,湖南衡阳421001

出　　处：《中国现代医学杂志》2009年第20期3067-3070,3074,共5页China Journal of Modern Medicine

基　　金：湖南省衡阳市科技厅基金(No:2005KS01-020)

摘　　要：目的观察Canstatin基因在结肠癌组织中的表达及其重组蛋白抗结肠癌作用。方法采用RT-PCR检测40例结肠癌癌组织、相应的癌旁组织和远癌组织中Canstatin mRNA的表达情况。PCR产物经凝胶电泳,通过各组织中Canstatin与GAPDH条带的灰度值之比,半定量分析Canstatin mRNA的表达水平。建立结肠癌SW480裸鼠异种移植瘤模型,绘制移植瘤生长曲线,计算经重组Canstatin蛋白治疗后的肿瘤抑制率。结果癌组织中Canstatin mRNA表达水平(0.44±0.17)低于癌旁组织(0.59±0.14)和远癌组织(0.64±0.21)。随着肿瘤分化程度的升高,Canstatin mRNA在癌组织中的表达也增加(P<0.05),而在临床病理分期(Dukes),随着分期升高,Canstatin mRNA在结肠癌组织中表达明显减少(P<0.05),但与淋巴结转移和病理类型均无统计学差异(P>0.05);试验组肿瘤的生长明显受到抑制,瘤质量明显低于对照组(P<0.01)。低、中、高浓度Canstatin治疗组抑瘤作用较对照组明显增强(P<0.05),其瘤重抑制率分别为30.02%、52.98%和58.00%。结论Canstatin mRNA在结肠癌组织中低表达,重组人Canstatin蛋白能有效抑制人结肠癌生长,无明显毒副作用,为结肠癌治疗提供了新的有效治疗方法。[ Objective ] To study the expression and significance of Canstatin gene in human colorectal carcinoma and the anti-tumor effects of the recombinant Canstatin protein. [Methods] The expression of eanstatin gene was detected by RT-PCR in 40 cases of colorectal cancer tissues, matched para-cancer tissue and tissue 5 cm away from tumor margin. PCR products were tested by gel eleetrophoresis. The expression of Canstatin gene in three kinds oftissues were semi-quantified by calculating the ratio of the grey value of canstatin bands and that of GAPDH bands. Trail of human colon carcinoma xenografts in nude mouse model was used to draw the transplant tumor growth curve and test inhibition rate of the recombinant canstatin protein on human colon carcinoma. [ Results ] The expression level of canstatin mRNA in cancer tissue （0.44±0.17） was lower than that in para-eancer tissue （0.59±0.14） and tissue 5 cm away from tumor margin （0.64±0.21）. Higher expression level of canstatin mRNA was correlated with better tumor differentiation （P 〈0.05）. Relationship was also observed between the expression of canstatin mRNA and dukes, stages, but not lymph node metastasis and histopathological types （P 〉0.05）. In every treatment group tumor growth was suppressed significantly. Intraperitoneal injection of eanstatin low-dose group, canstatin meal-dose group, canstatin high-dose group, resulted in a significant inhibition of the growth of SW480 in vivo compare with that of control group （P 〈0.05）. Its tumor suppression rate was 30.02%, 52.98% and 58.00%. [ Conclusion] The expression of canstatin mRNA in colorectal cancer tissues were reduced. Recombinant human Canstatin protein effectively retards the growth of colon cancer without remarkable adverse effects. In all, for the treatment of colon cancer provides a new and effective treatment.

关 键 词：结肠癌 逆转录聚合酶链反应 CANSTATIN Ⅳ型胶原 移植瘤 

分 类 号：R731.3[医药卫生—肿瘤]